Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21778 | 3' | -51.7 | NC_004813.1 | + | 44240 | 0.71 | 0.657472 |
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Target: 5'- -gGUGGAUGCGGAGCAguuUgGUggcCAGCa -3' miRNA: 3'- ggCGCCUAUGUCUCGU---GgCAaa-GUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 44354 | 0.72 | 0.61292 |
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Target: 5'- aCCGCGGAUAUGGuAGUucACCGUcaUCuGCu -3' miRNA: 3'- -GGCGCCUAUGUC-UCG--UGGCAa-AGuCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 38772 | 0.73 | 0.557676 |
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Target: 5'- gCGCGGGcaaggGCAGccguGUACCGUgcUCAGCa -3' miRNA: 3'- gGCGCCUa----UGUCu---CGUGGCAa-AGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 28264 | 0.74 | 0.49345 |
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Target: 5'- aCUGCGGAUuguGGAuGCACCGgg-CAGCc -3' miRNA: 3'- -GGCGCCUAug-UCU-CGUGGCaaaGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 31335 | 0.76 | 0.367031 |
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Target: 5'- aUGCGGAUGCAGAgGCGgCGUUUggacaguuuguugCAGCa -3' miRNA: 3'- gGCGCCUAUGUCU-CGUgGCAAA-------------GUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 30042 | 0.8 | 0.24611 |
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Target: 5'- cCUGUGGGgcagugUACGGuGCGCCGggUCAGCg -3' miRNA: 3'- -GGCGCCU------AUGUCuCGUGGCaaAGUCG- -5' |
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| 21778 | 3' | -51.7 | NC_004813.1 | + | 45587 | 1.16 | 0.000978 |
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Target: 5'- aCCGCGGAUACAGAGCACCGUUUCAGCg -3' miRNA: 3'- -GGCGCCUAUGUCUCGUGGCAAAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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