Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21782 | 3' | -55.2 | NC_004813.1 | + | 35388 | 0.66 | 0.734873 |
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Target: 5'- gCGACCGGCGGG-CGUCuggaaAUUCUCUg- -3' miRNA: 3'- aGCUGGCCGUCUaGUAG-----UAAGGGGgc -5' |
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| 21782 | 3' | -55.2 | NC_004813.1 | + | 32353 | 0.67 | 0.692434 |
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Target: 5'- aCGACagaGGCGGG-CAaUAUcCCCCCGg -3' miRNA: 3'- aGCUGg--CCGUCUaGUaGUAaGGGGGC- -5' |
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| 21782 | 3' | -55.2 | NC_004813.1 | + | 29291 | 0.69 | 0.572971 |
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Target: 5'- ---cCCaGCAGAUCAUCcaggCCCCCGc -3' miRNA: 3'- agcuGGcCGUCUAGUAGuaa-GGGGGC- -5' |
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| 21782 | 3' | -55.2 | NC_004813.1 | + | 45705 | 0.69 | 0.562222 |
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Target: 5'- cUGACCGuCAGGgug-UAUUCCCCCGg -3' miRNA: 3'- aGCUGGCcGUCUaguaGUAAGGGGGC- -5' |
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| 21782 | 3' | -55.2 | NC_004813.1 | + | 35165 | 0.75 | 0.266904 |
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Target: 5'- aCGACCuGCAGAUauugcCAUUCCCCCu -3' miRNA: 3'- aGCUGGcCGUCUAgua--GUAAGGGGGc -5' |
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| 21782 | 3' | -55.2 | NC_004813.1 | + | 46729 | 1.1 | 0.000985 |
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Target: 5'- uUCGACCGGCAGAUCAUCAUUCCCCCGg -3' miRNA: 3'- -AGCUGGCCGUCUAGUAGUAAGGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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