miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21783 5' -49.2 NC_004813.1 + 48348 1.06 0.004873
Target:  5'- uCAUCACAAAGUGCAGCAGAAGCUGAAu -3'
miRNA:   3'- -GUAGUGUUUCACGUCGUCUUCGACUU- -5'
21783 5' -49.2 NC_004813.1 + 45330 0.67 0.887132
Target:  5'- cCAUCACGGcGGUGCAGCAcguaccggaaaaaGAAGCcaucgUGGAu -3'
miRNA:   3'- -GUAGUGUU-UCACGUCGU-------------CUUCG-----ACUU- -5'
21783 5' -49.2 NC_004813.1 + 41667 0.66 0.929623
Target:  5'- aAUC-CGGAG-GCcgacccggAGCAGGAGCUGAc -3'
miRNA:   3'- gUAGuGUUUCaCG--------UCGUCUUCGACUu -5'
21783 5' -49.2 NC_004813.1 + 46044 0.66 0.946198
Target:  5'- gCAUCuggcuCAGGG-GCuGUGGGAGCUGAu -3'
miRNA:   3'- -GUAGu----GUUUCaCGuCGUCUUCGACUu -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.