Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21785 | 3' | -39.8 | NC_004813.1 | + | 40311 | 0.66 | 1 |
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Target: 5'- --gGAcUGGCAGAAAG--GGCuGCAGGAg -3' miRNA: 3'- aaaCU-ACUGUUUUUUaaUCG-CGUUCU- -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 48102 | 0.66 | 1 |
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Target: 5'- -cUGAUgGugGAAGAggUGGCGCGu-- -3' miRNA: 3'- aaACUA-CugUUUUUuaAUCGCGUucu -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 35243 | 0.66 | 1 |
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Target: 5'- --cGGUG-CGGAAA--UGGCGCAGGc -3' miRNA: 3'- aaaCUACuGUUUUUuaAUCGCGUUCu -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 43293 | 0.67 | 0.999999 |
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Target: 5'- --cGGUGACGGGAAggUGGUgguuaucgggcgGCAGGGg -3' miRNA: 3'- aaaCUACUGUUUUUuaAUCG------------CGUUCU- -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 26230 | 0.68 | 0.999993 |
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Target: 5'- gUUGAUGACAAugcagaagcagcuGGAAggGGCaCAGGAc -3' miRNA: 3'- aAACUACUGUU-------------UUUUaaUCGcGUUCU- -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 15456 | 0.69 | 0.999955 |
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Target: 5'- aUUGAUGGCGAucGGgcagGGCGUcAGAa -3' miRNA: 3'- aAACUACUGUUuuUUaa--UCGCGuUCU- -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 6249 | 0.74 | 0.994618 |
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Target: 5'- gUUUGAUGACGAuguuGAGUUucaggAGCGCAuggcAGAa -3' miRNA: 3'- -AAACUACUGUUu---UUUAA-----UCGCGU----UCU- -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 50945 | 0.82 | 0.804369 |
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Target: 5'- -cUGA-GACAGAGcAUUAGCGCAAGGu -3' miRNA: 3'- aaACUaCUGUUUUuUAAUCGCGUUCU- -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 55812 | 0.82 | 0.804369 |
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Target: 5'- -cUGA-GACAGAGuAUUAGCGCAAGGu -3' miRNA: 3'- aaACUaCUGUUUUuUAAUCGCGUUCU- -5' |
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| 21785 | 3' | -39.8 | NC_004813.1 | + | 55692 | 1.1 | 0.039651 |
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Target: 5'- gUUUGAUGACAAAAAAUUAGCGCAAGAg -3' miRNA: 3'- -AAACUACUGUUUUUUAAUCGCGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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