Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21799 | 3' | -40.8 | NC_004827.1 | + | 30177 | 0.67 | 0.999901 |
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Target: 5'- uGAGCgCUAUUUGCCGUcuauUAAAGUUu -3' miRNA: 3'- gUUUG-GGUAAACGGCGuu--AUUUUAAu -5' |
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| 21799 | 3' | -40.8 | NC_004827.1 | + | 32217 | 0.67 | 0.999881 |
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Target: 5'- aCAAGCCCGUcgcGCaacaccgauucaaacUGCGGUGAAAUUAg -3' miRNA: 3'- -GUUUGGGUAaa-CG---------------GCGUUAUUUUAAU- -5' |
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| 21799 | 3' | -40.8 | NC_004827.1 | + | 10548 | 0.69 | 0.999282 |
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Target: 5'- uGAGCCguUUUGUCaGCAAUGAAGa-- -3' miRNA: 3'- gUUUGGguAAACGG-CGUUAUUUUaau -5' |
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| 21799 | 3' | -40.8 | NC_004827.1 | + | 10732 | 0.71 | 0.995861 |
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Target: 5'- aAAGCCU-UUUGCCGcCAAUAAAAg-- -3' miRNA: 3'- gUUUGGGuAAACGGC-GUUAUUUUaau -5' |
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| 21799 | 3' | -40.8 | NC_004827.1 | + | 2010 | 0.76 | 0.908417 |
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Target: 5'- aUAAGCCCAUgcgGCCGCAAUc------ -3' miRNA: 3'- -GUUUGGGUAaa-CGGCGUUAuuuuaau -5' |
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| 21799 | 3' | -40.8 | NC_004827.1 | + | 10656 | 1.09 | 0.024452 |
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Target: 5'- uCAAACCCAUUUGCCGCAAUAAAAUUAa -3' miRNA: 3'- -GUUUGGGUAAACGGCGUUAUUUUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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