Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21799 | 5' | -47.3 | NC_004827.1 | + | 42318 | 0.66 | 0.966766 |
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Target: 5'- cAGCGUUUgaugUGUugggUGAUGAAACGGUg -3' miRNA: 3'- -UCGCAGAaguaACG----ACUGUUUUGCCG- -5' |
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| 21799 | 5' | -47.3 | NC_004827.1 | + | 21893 | 0.66 | 0.966766 |
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Target: 5'- -uUGUUUgaugggCGUUGCUGAUAAAGCGcGUu -3' miRNA: 3'- ucGCAGAa-----GUAACGACUGUUUUGC-CG- -5' |
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| 21799 | 5' | -47.3 | NC_004827.1 | + | 23312 | 0.67 | 0.962957 |
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Target: 5'- cGGUcaGUCcguUUCAggGUUGGCA-AGCGGCg -3' miRNA: 3'- -UCG--CAG---AAGUaaCGACUGUuUUGCCG- -5' |
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| 21799 | 5' | -47.3 | NC_004827.1 | + | 31279 | 0.69 | 0.900514 |
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Target: 5'- cGUGUCg--AUUGCuuaUGACGAAAcCGGCa -3' miRNA: 3'- uCGCAGaagUAACG---ACUGUUUU-GCCG- -5' |
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| 21799 | 5' | -47.3 | NC_004827.1 | + | 10581 | 0.73 | 0.712644 |
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Target: 5'- aGGCGUUuaauUUUAUUGC-GGCAAAugGGUu -3' miRNA: 3'- -UCGCAG----AAGUAACGaCUGUUUugCCG- -5' |
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| 21799 | 5' | -47.3 | NC_004827.1 | + | 10619 | 1.13 | 0.00256 |
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Target: 5'- uAGCGUCUUCAUUGCUGACAAAACGGCu -3' miRNA: 3'- -UCGCAGAAGUAACGACUGUUUUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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