Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 218 | 5' | -54.4 | AC_000008.1 | + | 9547 | 0.66 | 0.685163 |
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Target: 5'- cCCGUcauGuCCCGGuuaUGgGUUGGCGGGGg -3' miRNA: 3'- -GGCA---CuGGGUCug-AUgCGACUGCUCC- -5' |
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| 218 | 5' | -54.4 | AC_000008.1 | + | 16120 | 0.67 | 0.651745 |
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Target: 5'- aCCGUGgugcgcggaGCCCGGcGCUAUGCUaaaaugaagaGACGGcGGa -3' miRNA: 3'- -GGCAC---------UGGGUC-UGAUGCGA----------CUGCU-CC- -5' |
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| 218 | 5' | -54.4 | AC_000008.1 | + | 7635 | 0.67 | 0.618157 |
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Target: 5'- cCCGUGcucugaaaggGCCCAGuCUGCaa-GAUGAGGg -3' miRNA: 3'- -GGCAC----------UGGGUCuGAUGcgaCUGCUCC- -5' |
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| 218 | 5' | -54.4 | AC_000008.1 | + | 10379 | 0.68 | 0.595811 |
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Target: 5'- -gGUGGCCgGGGCUcCGggGGCGAGa -3' miRNA: 3'- ggCACUGGgUCUGAuGCgaCUGCUCc -5' |
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| 218 | 5' | -54.4 | AC_000008.1 | + | 12904 | 0.69 | 0.534142 |
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Target: 5'- aCC-UGAgCCAGGCUuucaaaaacuugcagGgGCUGugGGGGg -3' miRNA: 3'- -GGcACUgGGUCUGA---------------UgCGACugCUCC- -5' |
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| 218 | 5' | -54.4 | AC_000008.1 | + | 5344 | 0.69 | 0.497801 |
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Target: 5'- -gGUGcGCuCCGGGCUgcGCGCUGGCcAGGg -3' miRNA: 3'- ggCAC-UG-GGUCUGA--UGCGACUGcUCC- -5' |
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| 218 | 5' | -54.4 | AC_000008.1 | + | 11199 | 0.7 | 0.445508 |
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Target: 5'- gCCG-GGCCCGGcACUAC-CUGgacuuggaggaggGCGAGGg -3' miRNA: 3'- -GGCaCUGGGUC-UGAUGcGAC-------------UGCUCC- -5' |
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| 218 | 5' | -54.4 | AC_000008.1 | + | 2686 | 0.72 | 0.371008 |
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Target: 5'- aUGUGGCCgGGgguGCUugGCaugGACGGGGu -3' miRNA: 3'- gGCACUGGgUC---UGAugCGa--CUGCUCC- -5' |
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| 218 | 5' | -54.4 | AC_000008.1 | + | 30566 | 1.13 | 0.000448 |
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Target: 5'- aCCGUGACCCAGACUACGCUGACGAGGa -3' miRNA: 3'- -GGCACUGGGUCUGAUGCGACUGCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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