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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21806 | 3' | -47.8 | NC_004827.1 | + | 5419 | 0.66 | 0.956239 |
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Target: 5'- gUAUAACAUcaucACGCCGGUgauaauugcuauAAGUGCGGu -3' miRNA: 3'- -AUAUUGUAc---UGUGGCCG------------UUUACGCUg -5' |
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| 21806 | 3' | -47.8 | NC_004827.1 | + | 10309 | 0.67 | 0.9358 |
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Target: 5'- aGUGGCGUGAaagaguuCCGGCGGAacguUGCG-Ca -3' miRNA: 3'- aUAUUGUACUgu-----GGCCGUUU----ACGCuG- -5' |
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| 21806 | 3' | -47.8 | NC_004827.1 | + | 5849 | 0.68 | 0.886661 |
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Target: 5'- -uUAGCGUGACGguauaugucuucuCCGGU---UGCGGCa -3' miRNA: 3'- auAUUGUACUGU-------------GGCCGuuuACGCUG- -5' |
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| 21806 | 3' | -47.8 | NC_004827.1 | + | 4798 | 0.69 | 0.833986 |
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Target: 5'- -uUAGCuaucGGCGCCGGCGcGUGgGAUa -3' miRNA: 3'- auAUUGua--CUGUGGCCGUuUACgCUG- -5' |
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| 21806 | 3' | -47.8 | NC_004827.1 | + | 32826 | 1.1 | 0.003277 |
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Target: 5'- cUAUAACAUGACACCGGCAAAUGCGACg -3' miRNA: 3'- -AUAUUGUACUGUGGCCGUUUACGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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