Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21808 | 3' | -47.2 | NC_004827.1 | + | 5681 | 0.66 | 0.97888 |
Target: 5'- aCAAACUGGCGCG-UACUGAAg---- -3' miRNA: 3'- aGUUUGGCCGUGCgAUGGUUUauacu -5' |
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21808 | 3' | -47.2 | NC_004827.1 | + | 35962 | 0.66 | 0.969803 |
Target: 5'- uUCAGACCGGCAUccaCUugUAcaaaGAUAUGc -3' miRNA: 3'- -AGUUUGGCCGUGc--GAugGU----UUAUACu -5' |
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21808 | 3' | -47.2 | NC_004827.1 | + | 38738 | 0.67 | 0.958233 |
Target: 5'- uUCAAACCGGCAU----CCAGAU-UGAg -3' miRNA: 3'- -AGUUUGGCCGUGcgauGGUUUAuACU- -5' |
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21808 | 3' | -47.2 | NC_004827.1 | + | 34199 | 0.67 | 0.949017 |
Target: 5'- -gGAAUUGGuCGCGgUGCCAAGUAuuuUGAg -3' miRNA: 3'- agUUUGGCC-GUGCgAUGGUUUAU---ACU- -5' |
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21808 | 3' | -47.2 | NC_004827.1 | + | 31603 | 0.68 | 0.938543 |
Target: 5'- uUCAcuAUCGGUuaauuGCGCUGCCAcg-GUGAa -3' miRNA: 3'- -AGUu-UGGCCG-----UGCGAUGGUuuaUACU- -5' |
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21808 | 3' | -47.2 | NC_004827.1 | + | 40325 | 0.68 | 0.920412 |
Target: 5'- aUAAACCGGCACGCcucaAUCAuAUuUGGu -3' miRNA: 3'- aGUUUGGCCGUGCGa---UGGUuUAuACU- -5' |
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21808 | 3' | -47.2 | NC_004827.1 | + | 5909 | 0.69 | 0.906707 |
Target: 5'- aCAuauACCGuCACGCUaACgGAAUAUGAa -3' miRNA: 3'- aGUu--UGGCcGUGCGA-UGgUUUAUACU- -5' |
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21808 | 3' | -47.2 | NC_004827.1 | + | 40238 | 1.11 | 0.003618 |
Target: 5'- aUCAAACCGGCACGCUACCAAAUAUGAu -3' miRNA: 3'- -AGUUUGGCCGUGCGAUGGUUUAUACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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