Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
21809 | 5' | -57.5 | NC_004827.1 | + | 40403 | 0.66 | 0.466597 |
Target: 5'- uGCCGCGCcagGCUGgCGUGCAAUa-- -3' miRNA: 3'- uCGGCGUGaa-UGGCgGCGCGUUAgga -5' |
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21809 | 5' | -57.5 | NC_004827.1 | + | 4295 | 0.7 | 0.271658 |
Target: 5'- cGCCGUaaccuuguugcguuaACUcaccACCGCCGCGCGuuguUCCUu -3' miRNA: 3'- uCGGCG---------------UGAa---UGGCGGCGCGUu---AGGA- -5' |
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21809 | 5' | -57.5 | NC_004827.1 | + | 24221 | 0.72 | 0.212153 |
Target: 5'- cGCUGCAUUUAUCGCC-CGCGA-CCa -3' miRNA: 3'- uCGGCGUGAAUGGCGGcGCGUUaGGa -5' |
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21809 | 5' | -57.5 | NC_004827.1 | + | 42632 | 1.07 | 0.000558 |
Target: 5'- aAGCCGCACUUACCGCCGCGCAAUCCUc -3' miRNA: 3'- -UCGGCGUGAAUGGCGGCGCGUUAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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