miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
2181 5' -53.1 NC_001396.1 + 5227 0.66 0.22404
Target:  5'- gGCGAGA--GGGCCGcuAGCAgCGCCu- -3'
miRNA:   3'- aCGUUUUcaUCCGGCu-UCGU-GCGGcg -5'
2181 5' -53.1 NC_001396.1 + 1884 0.66 0.231164
Target:  5'- gGCAGGGGauGGCCuc-GCGUGCCGCu -3'
miRNA:   3'- aCGUUUUCauCCGGcuuCGUGCGGCG- -5'
2181 5' -53.1 NC_001396.1 + 527 0.67 0.197391
Target:  5'- cGCGAAG--GGGCCaGAauGGuCACGCCGa -3'
miRNA:   3'- aCGUUUUcaUCCGG-CU--UC-GUGCGGCg -5'
2181 5' -53.1 NC_001396.1 + 6193 0.7 0.124857
Target:  5'- aUGCAucuGUGGGCgucauGCAgGCCGCg -3'
miRNA:   3'- -ACGUuuuCAUCCGgcuu-CGUgCGGCG- -5'
2181 5' -53.1 NC_001396.1 + 4385 0.7 0.12909
Target:  5'- aGCGAGGGcAGGUCGuuuaucAGUcaGCGCCGUg -3'
miRNA:   3'- aCGUUUUCaUCCGGCu-----UCG--UGCGGCG- -5'
2181 5' -53.1 NC_001396.1 + 273 0.83 0.011689
Target:  5'- cUGCAAGGGUcaucAGGCCGAugaAGUaaGCGCCGCc -3'
miRNA:   3'- -ACGUUUUCA----UCCGGCU---UCG--UGCGGCG- -5'
2181 5' -53.1 NC_001396.1 + 790 1.13 0.000036
Target:  5'- uUGCAAAAGUAGGCCGAAGCACGCCGCg -3'
miRNA:   3'- -ACGUUUUCAUCCGGCUUCGUGCGGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.