miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21811 3' -40.1 NC_004828.1 + 4449 0.67 0.889594
Target:  5'- uGGGACGagguucgGAAUAGgaucccguGUcgGAGACGGCa -3'
miRNA:   3'- cUCUUGCa------UUUGUU--------UAuaUUCUGCCG- -5'
21811 3' -40.1 NC_004828.1 + 2138 0.72 0.625381
Target:  5'- cAGGugGUGAaucguGCAGGgagcAUAGGAUGGCu -3'
miRNA:   3'- cUCUugCAUU-----UGUUUa---UAUUCUGCCG- -5'
21811 3' -40.1 NC_004828.1 + 2522 0.73 0.566241
Target:  5'- cGAGGuCGUAAGCcuugucGUGUucuAAGGCGGCa -3'
miRNA:   3'- -CUCUuGCAUUUGuu----UAUA---UUCUGCCG- -5'
21811 3' -40.1 NC_004828.1 + 176 1.17 0.000578
Target:  5'- cGAGAACGUAAACAAAUAUAAGACGGCg -3'
miRNA:   3'- -CUCUUGCAUUUGUUUAUAUUCUGCCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.