miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21823 5' -46.1 NC_004831.2 + 15789 0.68 0.965491
Target:  5'- -uUGCAAGACugagaAGGAacucuaUAUGGCuguGCUUGGu -3'
miRNA:   3'- cuAUGUUCUG-----UCCU------AUAUCG---CGAACC- -5'
21823 5' -46.1 NC_004831.2 + 5075 0.72 0.836395
Target:  5'- cGAUGCAccGCAGGAUAUcuauggggcgguGGCGCaagUGGu -3'
miRNA:   3'- -CUAUGUucUGUCCUAUA------------UCGCGa--ACC- -5'
21823 5' -46.1 NC_004831.2 + 17068 0.73 0.76317
Target:  5'- aGAUAUuGGACAgGGAUGcccuUGGCGCUUGc -3'
miRNA:   3'- -CUAUGuUCUGU-CCUAU----AUCGCGAACc -5'
21823 5' -46.1 NC_004831.2 + 37024 0.75 0.670204
Target:  5'- --gACGAGACGGG-UGUAGCcauuguguucuuGCUUGGa -3'
miRNA:   3'- cuaUGUUCUGUCCuAUAUCG------------CGAACC- -5'
21823 5' -46.1 NC_004831.2 + 19272 1.11 0.004898
Target:  5'- uGAUACAAGACAGGAUAUAGCGCUUGGc -3'
miRNA:   3'- -CUAUGUUCUGUCCUAUAUCGCGAACC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.