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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 21823 | 5' | -46.1 | NC_004831.2 | + | 15789 | 0.68 | 0.965491 |
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Target: 5'- -uUGCAAGACugagaAGGAacucuaUAUGGCuguGCUUGGu -3' miRNA: 3'- cuAUGUUCUG-----UCCU------AUAUCG---CGAACC- -5' |
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| 21823 | 5' | -46.1 | NC_004831.2 | + | 5075 | 0.72 | 0.836395 |
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Target: 5'- cGAUGCAccGCAGGAUAUcuauggggcgguGGCGCaagUGGu -3' miRNA: 3'- -CUAUGUucUGUCCUAUA------------UCGCGa--ACC- -5' |
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| 21823 | 5' | -46.1 | NC_004831.2 | + | 17068 | 0.73 | 0.76317 |
|
Target: 5'- aGAUAUuGGACAgGGAUGcccuUGGCGCUUGc -3' miRNA: 3'- -CUAUGuUCUGU-CCUAU----AUCGCGAACc -5' |
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| 21823 | 5' | -46.1 | NC_004831.2 | + | 37024 | 0.75 | 0.670204 |
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Target: 5'- --gACGAGACGGG-UGUAGCcauuguguucuuGCUUGGa -3' miRNA: 3'- cuaUGUUCUGUCCuAUAUCG------------CGAACC- -5' |
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| 21823 | 5' | -46.1 | NC_004831.2 | + | 19272 | 1.11 | 0.004898 |
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Target: 5'- uGAUACAAGACAGGAUAUAGCGCUUGGc -3' miRNA: 3'- -CUAUGUUCUGUCCUAUAUCGCGAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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