Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21826 | 3' | -52 | NC_004831.2 | + | 19526 | 0.66 | 0.819737 |
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Target: 5'- uACCAGAaaaGCGagGGUCUCUUGCuggugugcgcuuGCUCa -3' miRNA: 3'- -UGGUCUa--CGUgaUCAGAGAACG------------UGGG- -5' |
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| 21826 | 3' | -52 | NC_004831.2 | + | 29654 | 0.66 | 0.779329 |
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Target: 5'- aAUCAGAUGCguaugugggguGCUGGugguuaccaaUCUCcUGCACCa -3' miRNA: 3'- -UGGUCUACG-----------UGAUC----------AGAGaACGUGGg -5' |
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| 21826 | 3' | -52 | NC_004831.2 | + | 21213 | 0.68 | 0.718435 |
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Target: 5'- uACCAGuu-CACUAGUgaaugaaucaccugcCUCUUGCAagcCCCa -3' miRNA: 3'- -UGGUCuacGUGAUCA---------------GAGAACGU---GGG- -5' |
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| 21826 | 3' | -52 | NC_004831.2 | + | 40277 | 0.68 | 0.668401 |
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Target: 5'- -aCAGAUGCACaAG-CUgUUGCugCUa -3' miRNA: 3'- ugGUCUACGUGaUCaGAgAACGugGG- -5' |
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| 21826 | 3' | -52 | NC_004831.2 | + | 28244 | 0.69 | 0.645352 |
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Target: 5'- cACCAagcaggaguuuGAUGCACUGGUUUCUcaauugGCAgCUa -3' miRNA: 3'- -UGGU-----------CUACGUGAUCAGAGAa-----CGUgGG- -5' |
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| 21826 | 3' | -52 | NC_004831.2 | + | 27749 | 1.11 | 0.001035 |
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Target: 5'- uACCAGAUGCACUAGUCUCUUGCACCCc -3' miRNA: 3'- -UGGUCUACGUGAUCAGAGAACGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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