Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21829 | 3' | -55 | NC_004831.2 | + | 27853 | 0.66 | 0.622914 |
Target: 5'- --gGCGGCGCAC-GCgCACACaUGCu- -3' miRNA: 3'- guaUGCCGCGUGaCG-GUGUGgAUGcu -5' |
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21829 | 3' | -55 | NC_004831.2 | + | 33283 | 0.66 | 0.621788 |
Target: 5'- --aAUGGCGCGg-GCCaacuccgGCACCUGCGc -3' miRNA: 3'- guaUGCCGCGUgaCGG-------UGUGGAUGCu -5' |
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21829 | 3' | -55 | NC_004831.2 | + | 19951 | 0.72 | 0.322434 |
Target: 5'- --cAgGGCGCACaagGCCACGCUUGCc- -3' miRNA: 3'- guaUgCCGCGUGa--CGGUGUGGAUGcu -5' |
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21829 | 3' | -55 | NC_004831.2 | + | 33832 | 1.09 | 0.000754 |
Target: 5'- aCAUACGGCGCACUGCCACACCUACGAu -3' miRNA: 3'- -GUAUGCCGCGUGACGGUGUGGAUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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