miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21830 3' -56.3 NC_004831.2 + 34271 1.09 0.000501
Target:  5'- aCACCAGCACCAGCCACUAAGUCGCCAa -3'
miRNA:   3'- -GUGGUCGUGGUCGGUGAUUCAGCGGU- -5'
21830 3' -56.3 NC_004831.2 + 31149 0.73 0.211004
Target:  5'- -cUCGGCACCAGCCACUGc--UGCCGu -3'
miRNA:   3'- guGGUCGUGGUCGGUGAUucaGCGGU- -5'
21830 3' -56.3 NC_004831.2 + 38478 0.69 0.39007
Target:  5'- -uCCGGCACagGGCCGCUAAGgagGCUAg -3'
miRNA:   3'- guGGUCGUGg-UCGGUGAUUCag-CGGU- -5'
21830 3' -56.3 NC_004831.2 + 22853 0.67 0.468334
Target:  5'- aGCCAGCuuCGGCUucagGCUAucaauGUCGCCc -3'
miRNA:   3'- gUGGUCGugGUCGG----UGAUu----CAGCGGu -5'
21830 3' -56.3 NC_004831.2 + 38277 0.66 0.564966
Target:  5'- gUugCAGUACCAGCauCACcguauuugauAGUUGCCAu -3'
miRNA:   3'- -GugGUCGUGGUCG--GUGau--------UCAGCGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.