Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21831 | 5' | -53.4 | NC_004831.2 | + | 36119 | 1.06 | 0.001264 |
Target: 5'- cUUAGCCUGACCACGCUGUGCUUCUACc -3' miRNA: 3'- -AAUCGGACUGGUGCGACACGAAGAUG- -5' |
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21831 | 5' | -53.4 | NC_004831.2 | + | 21445 | 0.7 | 0.444779 |
Target: 5'- -gGGCCUGugCuuGCUGUGCcauUUCUuguuGCa -3' miRNA: 3'- aaUCGGACugGugCGACACG---AAGA----UG- -5' |
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21831 | 5' | -53.4 | NC_004831.2 | + | 20927 | 0.68 | 0.554046 |
Target: 5'- -cAGaCCUGACaccuaucaGCGCUGUGCUggaauaUACa -3' miRNA: 3'- aaUC-GGACUGg-------UGCGACACGAag----AUG- -5' |
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21831 | 5' | -53.4 | NC_004831.2 | + | 14624 | 0.66 | 0.658283 |
Target: 5'- gUUAGCCUuACCAauCUGUGCUUUg-- -3' miRNA: 3'- -AAUCGGAcUGGUgcGACACGAAGaug -5' |
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21831 | 5' | -53.4 | NC_004831.2 | + | 13906 | 0.67 | 0.635011 |
Target: 5'- -gGGUCUG-CUGCGC-GUGCUUCgGCa -3' miRNA: 3'- aaUCGGACuGGUGCGaCACGAAGaUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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