miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21832 3' -50.9 NC_004831.2 + 36968 0.7 0.670587
Target:  5'- gGCCUauucaGCguaGGUUGAUGUAUAUCGACCc -3'
miRNA:   3'- -UGGA-----CGgc-CCAGCUAUAUGUAGUUGG- -5'
21832 3' -50.9 NC_004831.2 + 110 0.68 0.759675
Target:  5'- gGCCUGCCGGaGcgagCGAUAgggacGCGUguaGGCCg -3'
miRNA:   3'- -UGGACGGCC-Ca---GCUAUa----UGUAg--UUGG- -5'
21832 3' -50.9 NC_004831.2 + 43662 0.68 0.759675
Target:  5'- gGCCUGCCGGaGcgagCGAUAgggacGCGUguaGGCCg -3'
miRNA:   3'- -UGGACGGCC-Ca---GCUAUa----UGUAg--UUGG- -5'
21832 3' -50.9 NC_004831.2 + 37052 1.12 0.001342
Target:  5'- cACCUGCCGGGUCGAUAUACAUCAACCu -3'
miRNA:   3'- -UGGACGGCCCAGCUAUAUGUAGUUGG- -5'
21832 3' -50.9 NC_004831.2 + 33029 0.71 0.624684
Target:  5'- -aCUGCCGGGUCGGgaacccCAU-AGCCg -3'
miRNA:   3'- ugGACGGCCCAGCUauau--GUAgUUGG- -5'
21832 3' -50.9 NC_004831.2 + 20544 0.68 0.780784
Target:  5'- -gUUGCUGGGUCGAaUGUACGUagcGCUu -3'
miRNA:   3'- ugGACGGCCCAGCU-AUAUGUAgu-UGG- -5'
21832 3' -50.9 NC_004831.2 + 17190 0.66 0.890087
Target:  5'- aACCUGCU-GGUUGAUGcUugAaugCAACCu -3'
miRNA:   3'- -UGGACGGcCCAGCUAU-AugUa--GUUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.