Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21832 | 5' | -58.9 | NC_004831.2 | + | 11186 | 0.66 | 0.416534 |
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Target: 5'- cGGGaacaCGCCACUCuccaucauccuuguCGGACGUaGGCc -3' miRNA: 3'- aUCCg---GCGGUGAGuau-----------GCCUGCG-CCG- -5' |
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| 21832 | 5' | -58.9 | NC_004831.2 | + | 30713 | 0.67 | 0.365258 |
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Target: 5'- -cGGCUucuGCCACUUGUGCaaaGGuAgGCGGCa -3' miRNA: 3'- auCCGG---CGGUGAGUAUG---CC-UgCGCCG- -5' |
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| 21832 | 5' | -58.9 | NC_004831.2 | + | 11668 | 0.67 | 0.365258 |
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Target: 5'- ---aCCaCCACUCAUACGGAUGCGc- -3' miRNA: 3'- auccGGcGGUGAGUAUGCCUGCGCcg -5' |
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| 21832 | 5' | -58.9 | NC_004831.2 | + | 140 | 0.69 | 0.308497 |
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Target: 5'- gUAGGCCGCUugACagCGUGugUGGGCGUGGg -3' miRNA: 3'- -AUCCGGCGG--UGa-GUAU--GCCUGCGCCg -5' |
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| 21832 | 5' | -58.9 | NC_004831.2 | + | 43692 | 0.69 | 0.308497 |
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Target: 5'- gUAGGCCGCUugACagCGUGugUGGGCGUGGg -3' miRNA: 3'- -AUCCGGCGG--UGa-GUAU--GCCUGCGCCg -5' |
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| 21832 | 5' | -58.9 | NC_004831.2 | + | 15924 | 0.69 | 0.289199 |
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Target: 5'- -uGGCCGCUugGCUCAUAUGGcacaauucaaaggugACaucugGCGGCg -3' miRNA: 3'- auCCGGCGG--UGAGUAUGCC---------------UG-----CGCCG- -5' |
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| 21832 | 5' | -58.9 | NC_004831.2 | + | 36940 | 0.71 | 0.204589 |
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Target: 5'- -cGGCCuguGCCGCgucCGUAUGaGugGCGGCc -3' miRNA: 3'- auCCGG---CGGUGa--GUAUGC-CugCGCCG- -5' |
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| 21832 | 5' | -58.9 | NC_004831.2 | + | 37017 | 1.1 | 0.000247 |
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Target: 5'- aUAGGCCGCCACUCAUACGGACGCGGCa -3' miRNA: 3'- -AUCCGGCGGUGAGUAUGCCUGCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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