Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21833 | 5' | -50.3 | NC_004831.2 | + | 37850 | 1.05 | 0.003367 |
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Target: 5'- cUACCUAGGAAUACCUAUGACCUCCUAu -3' miRNA: 3'- -AUGGAUCCUUAUGGAUACUGGAGGAU- -5' |
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| 21833 | 5' | -50.3 | NC_004831.2 | + | 15752 | 0.69 | 0.694383 |
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Target: 5'- gACgUGGGGccaaGUacGCUUAUGACCUCCUu -3' miRNA: 3'- aUGgAUCCU----UA--UGGAUACUGGAGGAu -5' |
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| 21833 | 5' | -50.3 | NC_004831.2 | + | 47 | 0.67 | 0.815377 |
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Target: 5'- gGCgCUAGGGcgGCCUGUGGaggCCUGa -3' miRNA: 3'- aUG-GAUCCUuaUGGAUACUggaGGAU- -5' |
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| 21833 | 5' | -50.3 | NC_004831.2 | + | 43599 | 0.67 | 0.815377 |
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Target: 5'- gGCgCUAGGGcgGCCUGUGGaggCCUGa -3' miRNA: 3'- aUG-GAUCCUuaUGGAUACUggaGGAU- -5' |
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| 21833 | 5' | -50.3 | NC_004831.2 | + | 27999 | 0.66 | 0.853649 |
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Target: 5'- cGCCUAuGGGAUACCUAcccUGaACUUCgUGg -3' miRNA: 3'- aUGGAU-CCUUAUGGAU---AC-UGGAGgAU- -5' |
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| 21833 | 5' | -50.3 | NC_004831.2 | + | 23656 | 0.66 | 0.879572 |
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Target: 5'- gACCgAGGAAUaACCUAUGcCCUaUCUAc -3' miRNA: 3'- aUGGaUCCUUA-UGGAUACuGGA-GGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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