Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21834 | 3' | -53.5 | NC_004831.2 | + | 8863 | 0.65 | 0.730311 |
Target: 5'- cGUUAcGGUGCAgGCCACAAGGCaaucucugagGCUg -3' miRNA: 3'- -CGGU-CUACGU-UGGUGUUCCGgua-------CGA- -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 30189 | 0.66 | 0.71023 |
Target: 5'- aGCCu-AUGCAGCCuuCAAGGCUG-GCa -3' miRNA: 3'- -CGGucUACGUUGGu-GUUCCGGUaCGa -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 19239 | 0.66 | 0.71023 |
Target: 5'- uUguGGUGCAGCCAUgauAAGGCaggcuUGCUu -3' miRNA: 3'- cGguCUACGUUGGUG---UUCCGgu---ACGA- -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 21392 | 0.66 | 0.698951 |
Target: 5'- aGCaCAGGccCAGCaa-GAGGCCAUGCUc -3' miRNA: 3'- -CG-GUCUacGUUGgugUUCCGGUACGA- -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 28796 | 0.67 | 0.653252 |
Target: 5'- -gCAGAUGCGauauGCCAagcuGGGCCA-GCa -3' miRNA: 3'- cgGUCUACGU----UGGUgu--UCCGGUaCGa -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 39783 | 0.67 | 0.61871 |
Target: 5'- aGCCAGAUGCua-CACAauuAGGCU-UGUa -3' miRNA: 3'- -CGGUCUACGuugGUGU---UCCGGuACGa -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 29505 | 0.68 | 0.550242 |
Target: 5'- cGCCAGAUGCAuCCAgCAAuGCU-UGCUu -3' miRNA: 3'- -CGGUCUACGUuGGU-GUUcCGGuACGA- -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 12980 | 0.69 | 0.539026 |
Target: 5'- uGUCAGAUGCAGCUugauguACAAGGgCAauggaUGCc -3' miRNA: 3'- -CGGUCUACGUUGG------UGUUCCgGU-----ACGa -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 27622 | 0.71 | 0.432332 |
Target: 5'- cGCCGaGUGCAGuCUACugguGGGCCAagUGCUg -3' miRNA: 3'- -CGGUcUACGUU-GGUGu---UCCGGU--ACGA- -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 19963 | 0.71 | 0.432332 |
Target: 5'- aGCUAGAgGCuacagGGCgCACAAGGCCAcGCUu -3' miRNA: 3'- -CGGUCUaCG-----UUG-GUGUUCCGGUaCGA- -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 20844 | 0.71 | 0.412482 |
Target: 5'- uGCCAucaGUGCAGCCcCAcGGGCCAUGgCUc -3' miRNA: 3'- -CGGUc--UACGUUGGuGU-UCCGGUAC-GA- -5' |
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21834 | 3' | -53.5 | NC_004831.2 | + | 38160 | 1.09 | 0.000867 |
Target: 5'- uGCCAGAUGCAACCACAAGGCCAUGCUu -3' miRNA: 3'- -CGGUCUACGUUGGUGUUCCGGUACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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