Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21835 | 3' | -44.3 | NC_004831.2 | + | 23038 | 0.66 | 0.99734 |
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Target: 5'- ----cAUCUCCgAUgugGCUAUCCugauGCCg -3' miRNA: 3'- uaauuUGGAGGaUAa--UGAUAGGu---CGG- -5' |
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| 21835 | 3' | -44.3 | NC_004831.2 | + | 12143 | 0.67 | 0.994421 |
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Target: 5'- ---cAGCUUgcaCCUAUuuugUACUcgCCAGCCa -3' miRNA: 3'- uaauUUGGA---GGAUA----AUGAuaGGUCGG- -5' |
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| 21835 | 3' | -44.3 | NC_004831.2 | + | 34983 | 0.67 | 0.990855 |
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Target: 5'- uAUUAGGCCUCCUuacauCUuugaAUCC-GCCu -3' miRNA: 3'- -UAAUUUGGAGGAuaau-GA----UAGGuCGG- -5' |
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| 21835 | 3' | -44.3 | NC_004831.2 | + | 27208 | 0.68 | 0.989331 |
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Target: 5'- -aUAAGCCUCCcuUGUgGCUAUCaacuGCUg -3' miRNA: 3'- uaAUUUGGAGG--AUAaUGAUAGgu--CGG- -5' |
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| 21835 | 3' | -44.3 | NC_004831.2 | + | 1636 | 0.69 | 0.969093 |
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Target: 5'- -gUAAACCUCCUAUguguuaaccuCUAUCUaaucGGCUu -3' miRNA: 3'- uaAUUUGGAGGAUAau--------GAUAGG----UCGG- -5' |
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| 21835 | 3' | -44.3 | NC_004831.2 | + | 37823 | 0.7 | 0.965357 |
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Target: 5'- ---uAACUUCCUGUUAUUccUCGGCCu -3' miRNA: 3'- uaauUUGGAGGAUAAUGAuaGGUCGG- -5' |
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| 21835 | 3' | -44.3 | NC_004831.2 | + | 14067 | 0.76 | 0.709414 |
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Target: 5'- cAUUAAACCUCCUugAUUAaUAUUCAGCa -3' miRNA: 3'- -UAAUUUGGAGGA--UAAUgAUAGGUCGg -5' |
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| 21835 | 3' | -44.3 | NC_004831.2 | + | 40016 | 1.11 | 0.007225 |
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Target: 5'- cAUUAAACCUCCUAUUACUAUCCAGCCc -3' miRNA: 3'- -UAAUUUGGAGGAUAAUGAUAGGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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