Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21836 | 3' | -55.5 | NC_004831.2 | + | 18665 | 0.67 | 0.548761 |
Target: 5'- uGGUCUgucGGaaGCCCUUgUGGCCCGCu -3' miRNA: 3'- -CCGGAgu-CCaaUGGGAA-ACCGGGUGu -5' |
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21836 | 3' | -55.5 | NC_004831.2 | + | 33204 | 0.69 | 0.433622 |
Target: 5'- gGGCUuugcgCAGG-UGCCggagUUGGCCCGCGc -3' miRNA: 3'- -CCGGa----GUCCaAUGGga--AACCGGGUGU- -5' |
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21836 | 3' | -55.5 | NC_004831.2 | + | 5115 | 0.7 | 0.38609 |
Target: 5'- gGGUUUCAGGUUAacagCUUUGGCCCc-- -3' miRNA: 3'- -CCGGAGUCCAAUgg--GAAACCGGGugu -5' |
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21836 | 3' | -55.5 | NC_004831.2 | + | 43667 | 0.73 | 0.24528 |
Target: 5'- aGGCCUCcacAGGccGCCCUagcGCCCACAg -3' miRNA: 3'- -CCGGAG---UCCaaUGGGAaacCGGGUGU- -5' |
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21836 | 3' | -55.5 | NC_004831.2 | + | 115 | 0.73 | 0.24528 |
Target: 5'- aGGCCUCcacAGGccGCCCUagcGCCCACAg -3' miRNA: 3'- -CCGGAG---UCCaaUGGGAaacCGGGUGU- -5' |
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21836 | 3' | -55.5 | NC_004831.2 | + | 8940 | 0.75 | 0.18212 |
Target: 5'- aGCCUCAGaGaUUGCCUUgUGGCCUGCAc -3' miRNA: 3'- cCGGAGUC-C-AAUGGGAaACCGGGUGU- -5' |
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21836 | 3' | -55.5 | NC_004831.2 | + | 43709 | 1.1 | 0.000502 |
Target: 5'- aGGCCUCAGGUUACCCUUUGGCCCACAc -3' miRNA: 3'- -CCGGAGUCCAAUGGGAAACCGGGUGU- -5' |
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21836 | 3' | -55.5 | NC_004831.2 | + | 157 | 1.1 | 0.000502 |
Target: 5'- aGGCCUCAGGUUACCCUUUGGCCCACAc -3' miRNA: 3'- -CCGGAGUCCAAUGGGAAACCGGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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