Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21839 | 5' | -50.5 | NC_004831.2 | + | 33320 | 1.11 | 0.001322 |
Target: 5'- gGACAGCACAAGGUGAGCUACUUGAGCc -3' miRNA: 3'- -CUGUCGUGUUCCACUCGAUGAACUCG- -5' |
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21839 | 5' | -50.5 | NC_004831.2 | + | 26188 | 0.72 | 0.554594 |
Target: 5'- aGGCAGUACu-GGUGuGUauCUUGAGCg -3' miRNA: 3'- -CUGUCGUGuuCCACuCGauGAACUCG- -5' |
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21839 | 5' | -50.5 | NC_004831.2 | + | 35036 | 0.69 | 0.68188 |
Target: 5'- uGCAGCACGaacugucAGGUGcAGUUACUauAGCu -3' miRNA: 3'- cUGUCGUGU-------UCCAC-UCGAUGAacUCG- -5' |
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21839 | 5' | -50.5 | NC_004831.2 | + | 19784 | 0.67 | 0.8341 |
Target: 5'- aGGCAGC-UAAGGUuccAGCacaacaACUUGAGCg -3' miRNA: 3'- -CUGUCGuGUUCCAc--UCGa-----UGAACUCG- -5' |
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21839 | 5' | -50.5 | NC_004831.2 | + | 15569 | 0.66 | 0.84349 |
Target: 5'- uGAUAGUAcCGGGGUG-GC-ACUUGcAGCc -3' miRNA: 3'- -CUGUCGU-GUUCCACuCGaUGAAC-UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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