miRNA: 3'- -UAUG-CGAGA--GUCUCGagcGUCCUUCG- -5'
miRNA: 3'- -UAUGCGaGaGUCUCGaG-CGUCCuuCG- -5'
miRNA: 3'- -UAugCGa----GAG---UCUCGAGCGUCcUUCG- -5'
miRNA: 3'- -UAUGCGAGAG--UCUCgAGCgUCCUUCG- -5'
miRNA: 3'- -UAUGCGAGAGUCUCGAGCGUCCUUCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21840 | 5' | -54.1 | NC_004902.1 | + | 23546 | 0.66 | 0.693638 |
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Target: 5'- gGUACuGCUCUggUGGAGCcauCAGGGAGCu -3' miRNA: 3'- -UAUG-CGAGA--GUCUCGagcGUCCUUCG- -5' |
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| 21840 | 5' | -54.1 | NC_004902.1 | + | 19493 | 0.68 | 0.545861 |
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Target: 5'- aAUAUGUaC-CAGAGC-CaGCAGGggGCa -3' miRNA: 3'- -UAUGCGaGaGUCUCGaG-CGUCCuuCG- -5' |
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| 21840 | 5' | -54.1 | NC_004902.1 | + | 5055 | 0.69 | 0.512755 |
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Target: 5'- uGUugGCggcaaCUCuaaAGAGCUCGCAG-AAGUg -3' miRNA: 3'- -UAugCGa----GAG---UCUCGAGCGUCcUUCG- -5' |
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| 21840 | 5' | -54.1 | NC_004902.1 | + | 11477 | 0.81 | 0.085484 |
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Target: 5'- gAUACGCUCUCaaAGAGgUCGgAGGAGGUg -3' miRNA: 3'- -UAUGCGAGAG--UCUCgAGCgUCCUUCG- -5' |
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| 21840 | 5' | -54.1 | NC_004902.1 | + | 282 | 1.11 | 0.000596 |
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Target: 5'- gAUACGCUCUCAGAGCUCGCAGGAAGCg -3' miRNA: 3'- -UAUGCGAGAGUCUCGAGCGUCCUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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