Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21841 | 3' | -52.1 | NC_004902.1 | + | 34857 | 0.66 | 0.797112 |
Target: 5'- cGGUcCUGuaGACCAaauccugGCAGGCAcGCu -3' miRNA: 3'- aCCAuGACggCUGGUa------CGUUCGUaUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 1369 | 0.66 | 0.797111 |
Target: 5'- aGG-GCUGUCGACguUGUggGC-UGCc -3' miRNA: 3'- aCCaUGACGGCUGguACGuuCGuAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 26913 | 0.66 | 0.776287 |
Target: 5'- aGGUccaAUUGCaGcCCAUGCAGGCcUGCc -3' miRNA: 3'- aCCA---UGACGgCuGGUACGUUCGuAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 19388 | 0.66 | 0.776287 |
Target: 5'- uUGuUGCUGCCu-CUggGCAGGUAUACa -3' miRNA: 3'- -ACcAUGACGGcuGGuaCGUUCGUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 28469 | 0.66 | 0.776287 |
Target: 5'- cUGGUcaACUGagGAUUuauuGUGCAAGCGUACc -3' miRNA: 3'- -ACCA--UGACggCUGG----UACGUUCGUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 30126 | 0.67 | 0.743835 |
Target: 5'- ---gGCUGCCu-CCGUGCA-GUAUGCa -3' miRNA: 3'- accaUGACGGcuGGUACGUuCGUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 10023 | 0.68 | 0.664329 |
Target: 5'- uUGGUAgaGCUuACCAUGCA-GCAUcACc -3' miRNA: 3'- -ACCAUgaCGGcUGGUACGUuCGUA-UG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 39844 | 0.69 | 0.617866 |
Target: 5'- gUGGUGCUGCCuacGCCAU-CAcAGcCAUGCa -3' miRNA: 3'- -ACCAUGACGGc--UGGUAcGU-UC-GUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 25341 | 0.69 | 0.617866 |
Target: 5'- cGGUacacuGCUGCCacgcGACCAcacugGCAAGgGUGCu -3' miRNA: 3'- aCCA-----UGACGG----CUGGUa----CGUUCgUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 39439 | 0.7 | 0.548785 |
Target: 5'- aGGUGCUgGCUaGGCCAUGagccAGUAUGCa -3' miRNA: 3'- aCCAUGA-CGG-CUGGUACgu--UCGUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 33391 | 0.71 | 0.504062 |
Target: 5'- aUGGUggcaGCUGCaaaGACCAUGCAGuaaaauCAUGCu -3' miRNA: 3'- -ACCA----UGACGg--CUGGUACGUUc-----GUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 25398 | 0.71 | 0.493121 |
Target: 5'- gGGUGCUGCC--CCA-GC-AGCGUGCu -3' miRNA: 3'- aCCAUGACGGcuGGUaCGuUCGUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 3471 | 0.72 | 0.437118 |
Target: 5'- aGGUGCUGCCGuaaaugcagaugcgAUCAUGCGAcuGgAUGCa -3' miRNA: 3'- aCCAUGACGGC--------------UGGUACGUU--CgUAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 5528 | 0.74 | 0.344887 |
Target: 5'- uUGGUAauCUGUCGGCCGaagUGCAGGC-UGCc -3' miRNA: 3'- -ACCAU--GACGGCUGGU---ACGUUCGuAUG- -5' |
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21841 | 3' | -52.1 | NC_004902.1 | + | 949 | 1.09 | 0.00126 |
Target: 5'- cUGGUACUGCCGACCAUGCAAGCAUACc -3' miRNA: 3'- -ACCAUGACGGCUGGUACGUUCGUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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