Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21842 | 5' | -53.9 | NC_004902.1 | + | 26598 | 0.66 | 0.737993 |
Target: 5'- aGCUCuuggcUGAGgucGGCAGCCUGCAAUGc -3' miRNA: 3'- -CGGGu----AUUCaucCCGUCGGGUGUUGC- -5' |
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21842 | 5' | -53.9 | NC_004902.1 | + | 4148 | 0.66 | 0.727112 |
Target: 5'- cGCCUucAAGcAGGGguGCCCGaccugccuCAACa -3' miRNA: 3'- -CGGGuaUUCaUCCCguCGGGU--------GUUGc -5' |
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21842 | 5' | -53.9 | NC_004902.1 | + | 199 | 0.66 | 0.716129 |
Target: 5'- gGCCUu--AGcAGGGC-GCCCACGcCGu -3' miRNA: 3'- -CGGGuauUCaUCCCGuCGGGUGUuGC- -5' |
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21842 | 5' | -53.9 | NC_004902.1 | + | 3957 | 0.66 | 0.682703 |
Target: 5'- gGCCCAggccgccAGGGCGuUCCGCAAUGg -3' miRNA: 3'- -CGGGUauuca--UCCCGUcGGGUGUUGC- -5' |
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21842 | 5' | -53.9 | NC_004902.1 | + | 29789 | 0.68 | 0.603449 |
Target: 5'- uGCUCAacaAAGUGcGGC-GCCCACAACu -3' miRNA: 3'- -CGGGUa--UUCAUcCCGuCGGGUGUUGc -5' |
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21842 | 5' | -53.9 | NC_004902.1 | + | 24338 | 0.69 | 0.558495 |
Target: 5'- uGCUUAUGAaUGGGGCGGCCaucacugguUACGAUGg -3' miRNA: 3'- -CGGGUAUUcAUCCCGUCGG---------GUGUUGC- -5' |
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21842 | 5' | -53.9 | NC_004902.1 | + | 1390 | 0.69 | 0.510213 |
Target: 5'- uGCCCuacuuauGGGCAguuucGCCCACAACu -3' miRNA: 3'- -CGGGuauucauCCCGU-----CGGGUGUUGc -5' |
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21842 | 5' | -53.9 | NC_004902.1 | + | 1450 | 1.1 | 0.000766 |
Target: 5'- uGCCCAUAAGUAGGGCAGCCCACAACGu -3' miRNA: 3'- -CGGGUAUUCAUCCCGUCGGGUGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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