Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21843 | 3' | -56.9 | NC_004902.1 | + | 35643 | 0.66 | 0.514351 |
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Target: 5'- gCACUC-UGG-CAGCUCUGCCgUGaucUGCUg -3' miRNA: 3'- -GUGAGuAUCgGUCGAGGCGG-AC---ACGA- -5' |
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| 21843 | 3' | -56.9 | NC_004902.1 | + | 35112 | 0.67 | 0.4722 |
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Target: 5'- aCACaCAacaAGCCuGCUCCGCCUGgGUc -3' miRNA: 3'- -GUGaGUa--UCGGuCGAGGCGGACaCGa -5' |
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| 21843 | 3' | -56.9 | NC_004902.1 | + | 18773 | 0.67 | 0.461934 |
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Target: 5'- uGCUgGUA--UAGCUgCGCCUGUGCa -3' miRNA: 3'- gUGAgUAUcgGUCGAgGCGGACACGa -5' |
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| 21843 | 3' | -56.9 | NC_004902.1 | + | 23623 | 0.67 | 0.441762 |
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Target: 5'- cCugUCcacuGCCAGCcgcaccagaUCUGCCUGUGUg -3' miRNA: 3'- -GugAGuau-CGGUCG---------AGGCGGACACGa -5' |
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| 21843 | 3' | -56.9 | NC_004902.1 | + | 1137 | 0.68 | 0.412467 |
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Target: 5'- aGCUUGU-GCCAGCUCaCGCCUuggaugccugagGUGUUg -3' miRNA: 3'- gUGAGUAuCGGUCGAG-GCGGA------------CACGA- -5' |
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| 21843 | 3' | -56.9 | NC_004902.1 | + | 5729 | 0.73 | 0.186093 |
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Target: 5'- gCACUgAUGcuGCCGGCUCUGCUgGUGCa -3' miRNA: 3'- -GUGAgUAU--CGGUCGAGGCGGaCACGa -5' |
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| 21843 | 3' | -56.9 | NC_004902.1 | + | 1793 | 1.08 | 0.000466 |
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Target: 5'- aCACUCAUAGCCAGCUCCGCCUGUGCUg -3' miRNA: 3'- -GUGAGUAUCGGUCGAGGCGGACACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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