miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21845 3' -57.8 NC_004902.1 + 10645 0.66 0.497517
Target:  5'- --aGCUGCGCGaaGCCGCCUccguaaacguggcaGCAgCCAc -3'
miRNA:   3'- guaCGACGUGUagUGGCGGG--------------CGU-GGU- -5'
21845 3' -57.8 NC_004902.1 + 34211 0.66 0.490211
Target:  5'- uGUGCUGUACugaugCACUGacaGCACCAa -3'
miRNA:   3'- gUACGACGUGua---GUGGCgggCGUGGU- -5'
21845 3' -57.8 NC_004902.1 + 22157 0.66 0.46353
Target:  5'- gGUGCUGCACG-CAucUCGugcgaaauaaauguaCCUGCACCAg -3'
miRNA:   3'- gUACGACGUGUaGU--GGC---------------GGGCGUGGU- -5'
21845 3' -57.8 NC_004902.1 + 30970 0.66 0.459492
Target:  5'- uGUGCUcCACGUCGCCaagaaaugCUGCACCAa -3'
miRNA:   3'- gUACGAcGUGUAGUGGcg------GGCGUGGU- -5'
21845 3' -57.8 NC_004902.1 + 17242 0.66 0.459492
Target:  5'- aCAUacCUGCGCAg-ACCGCCCGaCACUu -3'
miRNA:   3'- -GUAc-GACGUGUagUGGCGGGC-GUGGu -5'
21845 3' -57.8 NC_004902.1 + 6985 0.67 0.420191
Target:  5'- --gGCUGUauuGCAaCGCCGCCaggaugGCACCGa -3'
miRNA:   3'- guaCGACG---UGUaGUGGCGGg-----CGUGGU- -5'
21845 3' -57.8 NC_004902.1 + 22819 0.67 0.410687
Target:  5'- --gGCUGCugAUCGCCaccaCCCcCGCCGc -3'
miRNA:   3'- guaCGACGugUAGUGGc---GGGcGUGGU- -5'
21845 3' -57.8 NC_004902.1 + 925 0.69 0.315443
Target:  5'- -uUGCUGCACAcagggUCAUgGCCUucuaaGCACCu -3'
miRNA:   3'- guACGACGUGU-----AGUGgCGGG-----CGUGGu -5'
21845 3' -57.8 NC_004902.1 + 30089 0.71 0.244189
Target:  5'- -cUGCUG-ACAUCACUGCaCCGgGCCu -3'
miRNA:   3'- guACGACgUGUAGUGGCG-GGCgUGGu -5'
21845 3' -57.8 NC_004902.1 + 12371 0.72 0.197263
Target:  5'- aCGUGCUGCACc-CugCGCCUGUaggGCCGc -3'
miRNA:   3'- -GUACGACGUGuaGugGCGGGCG---UGGU- -5'
21845 3' -57.8 NC_004902.1 + 12081 0.74 0.155785
Target:  5'- -uUGCUGCACccaggccaccaucagAUaCACUGCCCGCACUc -3'
miRNA:   3'- guACGACGUG---------------UA-GUGGCGGGCGUGGu -5'
21845 3' -57.8 NC_004902.1 + 5658 1.08 0.000404
Target:  5'- aCAUGCUGCACAUCACCGCCCGCACCAu -3'
miRNA:   3'- -GUACGACGUGUAGUGGCGGGCGUGGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.