Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21845 | 5' | -57.8 | NC_004902.1 | + | 18485 | 0.66 | 0.508278 |
Target: 5'- --gUCUGUAuuCGCGCUGGa-GGCAcaGCu -3' miRNA: 3'- ucaAGACGUu-GCGCGACCgcCCGU--CG- -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 39770 | 0.66 | 0.497785 |
Target: 5'- ----aUGCAugGCuGUgaUGGCGuaGGCAGCa -3' miRNA: 3'- ucaagACGUugCG-CG--ACCGC--CCGUCG- -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 11125 | 0.66 | 0.497785 |
Target: 5'- uGGuUUCUGCuGCGCcuuuGUaGGCGGcGUAGCc -3' miRNA: 3'- -UC-AAGACGuUGCG----CGaCCGCC-CGUCG- -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 2230 | 0.66 | 0.456846 |
Target: 5'- aAGUa-UGCuACGCGaggcGGCGGGCGGa -3' miRNA: 3'- -UCAagACGuUGCGCga--CCGCCCGUCg -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 38223 | 0.67 | 0.412103 |
Target: 5'- aGGcUCUGCGGCGCGCUGaacgcacucguugucGCGcacCAGCa -3' miRNA: 3'- -UCaAGACGUUGCGCGAC---------------CGCcc-GUCG- -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 41295 | 0.68 | 0.380827 |
Target: 5'- --aUgUGCuuCGCGCgaucugGGCuGGCGGCg -3' miRNA: 3'- ucaAgACGuuGCGCGa-----CCGcCCGUCG- -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 28790 | 0.69 | 0.32161 |
Target: 5'- aAGUUCgccgGaaaGGCGCuCUGGCGGGaaugcCAGCg -3' miRNA: 3'- -UCAAGa---Cg--UUGCGcGACCGCCC-----GUCG- -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 5921 | 0.71 | 0.236682 |
Target: 5'- cAGUUucCUGCAcguauucaaucaGCGCGCUGuCGGuGCGGCc -3' miRNA: 3'- -UCAA--GACGU------------UGCGCGACcGCC-CGUCG- -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 36580 | 0.72 | 0.201742 |
Target: 5'- gGGUUCUGCGGCGUauGCccaugGGCcGGCAGg -3' miRNA: 3'- -UCAAGACGUUGCG--CGa----CCGcCCGUCg -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 37022 | 0.79 | 0.061419 |
Target: 5'- -----aGC-ACGCGCUGGCGGcGCAGCa -3' miRNA: 3'- ucaagaCGuUGCGCGACCGCC-CGUCG- -5' |
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21845 | 5' | -57.8 | NC_004902.1 | + | 5623 | 1.1 | 0.000293 |
Target: 5'- aAGUUCUGCAACGCGCUGGCGGGCAGCc -3' miRNA: 3'- -UCAAGACGUUGCGCGACCGCCCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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