Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21846 | 3' | -55.5 | NC_004902.1 | + | 28859 | 0.66 | 0.62113 |
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Target: 5'- aCGGGuaucCGGgAUGGGUGGcAGCUUGUu -3' miRNA: 3'- aGCCUc---GUCgUAUCCACCaUCGGACG- -5' |
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| 21846 | 3' | -55.5 | NC_004902.1 | + | 32856 | 0.67 | 0.576231 |
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Target: 5'- gUCGGAGCuGUAUuaccagauugacAGGUaugcaGGUggcaagcagaagGGCCUGCc -3' miRNA: 3'- -AGCCUCGuCGUA------------UCCA-----CCA------------UCGGACG- -5' |
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| 21846 | 3' | -55.5 | NC_004902.1 | + | 22732 | 0.67 | 0.521245 |
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Target: 5'- gCGGcuuagggcugucAGCGGCGgGGGUGGUGGCgaucaGCa -3' miRNA: 3'- aGCC------------UCGUCGUaUCCACCAUCGga---CG- -5' |
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| 21846 | 3' | -55.5 | NC_004902.1 | + | 12001 | 0.68 | 0.510481 |
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Target: 5'- gCGG-GCAGUGUAucuGaUGGUGGCCUGg -3' miRNA: 3'- aGCCuCGUCGUAU---CcACCAUCGGACg -5' |
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| 21846 | 3' | -55.5 | NC_004902.1 | + | 7875 | 0.68 | 0.475657 |
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Target: 5'- cUGGAuGCAuaugccauuuaccaGCgaGUGGGUGGUGuGCCUGUg -3' miRNA: 3'- aGCCU-CGU--------------CG--UAUCCACCAU-CGGACG- -5' |
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| 21846 | 3' | -55.5 | NC_004902.1 | + | 6863 | 0.69 | 0.418481 |
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Target: 5'- -gGGAGCAgGUGUGGGUaaagGGUgugGGCCUGg -3' miRNA: 3'- agCCUCGU-CGUAUCCA----CCA---UCGGACg -5' |
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| 21846 | 3' | -55.5 | NC_004902.1 | + | 12906 | 0.75 | 0.194371 |
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Target: 5'- ---aGGCGGUcUGGGUGGUGGCuCUGCg -3' miRNA: 3'- agccUCGUCGuAUCCACCAUCG-GACG- -5' |
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| 21846 | 3' | -55.5 | NC_004902.1 | + | 6198 | 1.13 | 0.000325 |
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Target: 5'- gUCGGAGCAGCAUAGGUGGUAGCCUGCg -3' miRNA: 3'- -AGCCUCGUCGUAUCCACCAUCGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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