miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
21846 5' -51.3 NC_004902.1 + 4459 0.66 0.827653
Target:  5'- gGGCAGACUGUCAgcacggagcaaaGCGCUGGUGu -3'
miRNA:   3'- gUCGUCUGGUAGUaag---------CGUGGUCAUg -5'
21846 5' -51.3 NC_004902.1 + 27663 0.66 0.814004
Target:  5'- gCAGCAGucuugcuCCAUCc--CGUugCGGUACc -3'
miRNA:   3'- -GUCGUCu------GGUAGuaaGCGugGUCAUG- -5'
21846 5' -51.3 NC_004902.1 + 5796 0.68 0.706435
Target:  5'- cCAGCAGAgCCGgca---GCAUCAGUGCu -3'
miRNA:   3'- -GUCGUCU-GGUaguaagCGUGGUCAUG- -5'
21846 5' -51.3 NC_004902.1 + 8234 0.69 0.683512
Target:  5'- aCGGCAGcuaauCCGUCugUUGCaACCGGUACc -3'
miRNA:   3'- -GUCGUCu----GGUAGuaAGCG-UGGUCAUG- -5'
21846 5' -51.3 NC_004902.1 + 15007 0.7 0.613828
Target:  5'- cCAGCAGGCCAuaUCAg--GCACUAG-GCa -3'
miRNA:   3'- -GUCGUCUGGU--AGUaagCGUGGUCaUG- -5'
21846 5' -51.3 NC_004902.1 + 6161 1.09 0.001641
Target:  5'- cCAGCAGACCAUCAUUCGCACCAGUACc -3'
miRNA:   3'- -GUCGUCUGGUAGUAAGCGUGGUCAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.