Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21846 | 5' | -51.3 | NC_004902.1 | + | 4459 | 0.66 | 0.827653 |
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Target: 5'- gGGCAGACUGUCAgcacggagcaaaGCGCUGGUGu -3' miRNA: 3'- gUCGUCUGGUAGUaag---------CGUGGUCAUg -5' |
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| 21846 | 5' | -51.3 | NC_004902.1 | + | 27663 | 0.66 | 0.814004 |
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Target: 5'- gCAGCAGucuugcuCCAUCc--CGUugCGGUACc -3' miRNA: 3'- -GUCGUCu------GGUAGuaaGCGugGUCAUG- -5' |
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| 21846 | 5' | -51.3 | NC_004902.1 | + | 5796 | 0.68 | 0.706435 |
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Target: 5'- cCAGCAGAgCCGgca---GCAUCAGUGCu -3' miRNA: 3'- -GUCGUCU-GGUaguaagCGUGGUCAUG- -5' |
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| 21846 | 5' | -51.3 | NC_004902.1 | + | 8234 | 0.69 | 0.683512 |
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Target: 5'- aCGGCAGcuaauCCGUCugUUGCaACCGGUACc -3' miRNA: 3'- -GUCGUCu----GGUAGuaAGCG-UGGUCAUG- -5' |
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| 21846 | 5' | -51.3 | NC_004902.1 | + | 15007 | 0.7 | 0.613828 |
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Target: 5'- cCAGCAGGCCAuaUCAg--GCACUAG-GCa -3' miRNA: 3'- -GUCGUCUGGU--AGUaagCGUGGUCaUG- -5' |
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| 21846 | 5' | -51.3 | NC_004902.1 | + | 6161 | 1.09 | 0.001641 |
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Target: 5'- cCAGCAGACCAUCAUUCGCACCAGUACc -3' miRNA: 3'- -GUCGUCUGGUAGUAAGCGUGGUCAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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