Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21847 | 3' | -52.3 | NC_004902.1 | + | 24377 | 0.66 | 0.800463 |
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Target: 5'- gGGGaUGAauUCu---UGCCGCUcggcauAGCCGGu -3' miRNA: 3'- -CCCaACU--AGuguuACGGCGA------UCGGCC- -5' |
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| 21847 | 3' | -52.3 | NC_004902.1 | + | 36543 | 0.67 | 0.769907 |
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Target: 5'- -uGUUGcaAggGCAGUGCCGCcagcAGCCGGu -3' miRNA: 3'- ccCAAC--UagUGUUACGGCGa---UCGGCC- -5' |
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| 21847 | 3' | -52.3 | NC_004902.1 | + | 5812 | 0.67 | 0.759403 |
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Target: 5'- cGGGgUGGUCA--GUGCaccaGCagAGCCGGc -3' miRNA: 3'- -CCCaACUAGUguUACGg---CGa-UCGGCC- -5' |
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| 21847 | 3' | -52.3 | NC_004902.1 | + | 32442 | 0.69 | 0.648814 |
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Target: 5'- uGG-UGGUgCACAAUGCCGUcagAGCCa- -3' miRNA: 3'- cCCaACUA-GUGUUACGGCGa--UCGGcc -5' |
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| 21847 | 3' | -52.3 | NC_004902.1 | + | 41549 | 0.69 | 0.64768 |
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Target: 5'- uGGUUGucaucuuGUCAgCAGUGCUGCU-GCCGa -3' miRNA: 3'- cCCAAC-------UAGU-GUUACGGCGAuCGGCc -5' |
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| 21847 | 3' | -52.3 | NC_004902.1 | + | 36629 | 0.7 | 0.614776 |
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Target: 5'- aGGccu-UCGCGcUGCCGCgggAGCCGGu -3' miRNA: 3'- cCCaacuAGUGUuACGGCGa--UCGGCC- -5' |
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| 21847 | 3' | -52.3 | NC_004902.1 | + | 33499 | 0.7 | 0.592147 |
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Target: 5'- uGGGUgacagaUGGUgGCAAggucugggauUGCCaGCUGGCCGa -3' miRNA: 3'- -CCCA------ACUAgUGUU----------ACGG-CGAUCGGCc -5' |
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| 21847 | 3' | -52.3 | NC_004902.1 | + | 6295 | 1.14 | 0.00072 |
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Target: 5'- cGGGUUGAUCACAAUGCCGCUAGCCGGa -3' miRNA: 3'- -CCCAACUAGUGUUACGGCGAUCGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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