Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21847 | 5' | -51.7 | NC_004902.1 | + | 12257 | 0.66 | 0.845182 |
Target: 5'- gGGCgauUGCagUGCAGauacaguCAUUGCAGGAgCGCu -3' miRNA: 3'- -CCGa--ACGg-ACGUU-------GUAGCGUUCU-GCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 30483 | 0.66 | 0.843369 |
Target: 5'- aGGCaUGCCaGCAACuauaggccuaucuugUGUAAGAgGCa -3' miRNA: 3'- -CCGaACGGaCGUUGua-------------GCGUUCUgCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 5877 | 0.66 | 0.817975 |
Target: 5'- uGGCUUcGUcaacaacgCUGCAAuCGUUGCugAAGGCGCc -3' miRNA: 3'- -CCGAA-CG--------GACGUU-GUAGCG--UUCUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 13272 | 0.67 | 0.798147 |
Target: 5'- --aUUGCaaGCAACGUUGCcacaggaaGGGGCGCa -3' miRNA: 3'- ccgAACGgaCGUUGUAGCG--------UUCUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 24149 | 0.67 | 0.798147 |
Target: 5'- aGGUgauugucaGCCUGgGcaAUgGCAAGACGCa -3' miRNA: 3'- -CCGaa------CGGACgUugUAgCGUUCUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 42187 | 0.67 | 0.787943 |
Target: 5'- uGGCgggGUCUGUGAaauCGUUGCAcuGGCGCu -3' miRNA: 3'- -CCGaa-CGGACGUU---GUAGCGUu-CUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 28877 | 0.67 | 0.777561 |
Target: 5'- uGGCa-GCUUGUu-CAUCGaGAGGCGCg -3' miRNA: 3'- -CCGaaCGGACGuuGUAGCgUUCUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 27101 | 0.67 | 0.756315 |
Target: 5'- cGGCUUGgCUGCuuuuUCGCAAGuagacuuaccaAUGCc -3' miRNA: 3'- -CCGAACgGACGuuguAGCGUUC-----------UGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 33473 | 0.68 | 0.723438 |
Target: 5'- uGGUUUGCCuUGUcc---CGCAGGGCGUa -3' miRNA: 3'- -CCGAACGG-ACGuuguaGCGUUCUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 242 | 0.68 | 0.712262 |
Target: 5'- cGCUUGCUucggGCGcuACAUCGguAGcggGCGCu -3' miRNA: 3'- cCGAACGGa---CGU--UGUAGCguUC---UGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 40118 | 0.7 | 0.59783 |
Target: 5'- aGuCUUGCCUGCGACAgccagguuacgUCGgGuagccugaaaGGGCGCa -3' miRNA: 3'- cC-GAACGGACGUUGU-----------AGCgU----------UCUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 25647 | 0.71 | 0.529942 |
Target: 5'- uGGCUacagacagcUGCCaagcagGCAAUuaaGCAAGGCGCa -3' miRNA: 3'- -CCGA---------ACGGa-----CGUUGuagCGUUCUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 12037 | 0.78 | 0.223049 |
Target: 5'- aGGC-UGUCUGCcauuaaGGCAgaggCGCAGGACGCu -3' miRNA: 3'- -CCGaACGGACG------UUGUa---GCGUUCUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 36779 | 0.79 | 0.194112 |
Target: 5'- uGCUUGCCUGCAuCAggGCAGaGCGCa -3' miRNA: 3'- cCGAACGGACGUuGUagCGUUcUGCG- -5' |
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21847 | 5' | -51.7 | NC_004902.1 | + | 6258 | 1.16 | 0.000563 |
Target: 5'- aGGCUUGCCUGCAACAUCGCAAGACGCa -3' miRNA: 3'- -CCGAACGGACGUUGUAGCGUUCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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