Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21850 | 5' | -55.2 | NC_004902.1 | + | 42625 | 0.66 | 0.617774 |
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Target: 5'- cGcCAGCCUGA-CAGCUAGUAacagCAGa -3' miRNA: 3'- uC-GUCGGACUaGUCGGUCGUaca-GUC- -5' |
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| 21850 | 5' | -55.2 | NC_004902.1 | + | 23549 | 0.66 | 0.617774 |
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Target: 5'- aGGCAGaUCUGGUgCGGCUGGCAgugGaCAGg -3' miRNA: 3'- -UCGUC-GGACUA-GUCGGUCGUa--CaGUC- -5' |
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| 21850 | 5' | -55.2 | NC_004902.1 | + | 16858 | 0.67 | 0.561035 |
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Target: 5'- aAGCGGCCUGAagauuguugaGGCgCAGguUGUaCAGg -3' miRNA: 3'- -UCGUCGGACUag--------UCG-GUCguACA-GUC- -5' |
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| 21850 | 5' | -55.2 | NC_004902.1 | + | 34086 | 0.67 | 0.554307 |
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Target: 5'- uGCAGCCaucaacacgucgcaGAUCAGCgCAGCggGUgAGg -3' miRNA: 3'- uCGUCGGa-------------CUAGUCG-GUCGuaCAgUC- -5' |
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| 21850 | 5' | -55.2 | NC_004902.1 | + | 42468 | 0.67 | 0.527655 |
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Target: 5'- cGUAGCCaGAUCAGagCGGCAUG-CAu -3' miRNA: 3'- uCGUCGGaCUAGUCg-GUCGUACaGUc -5' |
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| 21850 | 5' | -55.2 | NC_004902.1 | + | 35872 | 0.68 | 0.463375 |
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Target: 5'- uGC-GCCUGAUCgAGCCAGUc-GUCAu -3' miRNA: 3'- uCGuCGGACUAG-UCGGUCGuaCAGUc -5' |
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| 21850 | 5' | -55.2 | NC_004902.1 | + | 7266 | 0.68 | 0.463375 |
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Target: 5'- uGGCAcCCUGAUCAGU-AGCcUGUCAu -3' miRNA: 3'- -UCGUcGGACUAGUCGgUCGuACAGUc -5' |
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| 21850 | 5' | -55.2 | NC_004902.1 | + | 34343 | 0.79 | 0.085887 |
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Target: 5'- cGCAGCCUGGUaCAGCCGGCgccggauguuccucGUGUCGc -3' miRNA: 3'- uCGUCGGACUA-GUCGGUCG--------------UACAGUc -5' |
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| 21850 | 5' | -55.2 | NC_004902.1 | + | 6821 | 1.08 | 0.000689 |
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Target: 5'- cAGCAGCCUGAUCAGCCAGCAUGUCAGc -3' miRNA: 3'- -UCGUCGGACUAGUCGGUCGUACAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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