Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21851 | 3' | -56.5 | NC_004902.1 | + | 3605 | 0.66 | 0.56903 |
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Target: 5'- ----aUGGCAUUGCUGCAaUCgCCUGCGa -3' miRNA: 3'- cucuaACCGUAGUGGCGU-GG-GGACGU- -5' |
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| 21851 | 3' | -56.5 | NC_004902.1 | + | 24174 | 0.66 | 0.535998 |
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Target: 5'- aGGGUUGGCuugaucuuuaugaucUCGCCGUuugugccauugucGCCCUUGCGc -3' miRNA: 3'- cUCUAACCGu--------------AGUGGCG-------------UGGGGACGU- -5' |
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| 21851 | 3' | -56.5 | NC_004902.1 | + | 37109 | 0.67 | 0.4722 |
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Target: 5'- cGGGAgUGGU-UUACCGUACUCUUGCu -3' miRNA: 3'- -CUCUaACCGuAGUGGCGUGGGGACGu -5' |
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| 21851 | 3' | -56.5 | NC_004902.1 | + | 24406 | 0.67 | 0.451786 |
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Target: 5'- -----cGGUAUCGCUGUAUCCCUGg- -3' miRNA: 3'- cucuaaCCGUAGUGGCGUGGGGACgu -5' |
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| 21851 | 3' | -56.5 | NC_004902.1 | + | 26005 | 0.69 | 0.357633 |
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Target: 5'- uGAGAUUGGCGcUGCCGCaACCaaucuugugCCUGCc -3' miRNA: 3'- -CUCUAACCGUaGUGGCG-UGG---------GGACGu -5' |
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| 21851 | 3' | -56.5 | NC_004902.1 | + | 36775 | 0.7 | 0.32402 |
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Target: 5'- -uGAUgcUGGCGUgGCUGCugCCCUGg- -3' miRNA: 3'- cuCUA--ACCGUAgUGGCGugGGGACgu -5' |
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| 21851 | 3' | -56.5 | NC_004902.1 | + | 25424 | 0.71 | 0.284658 |
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Target: 5'- uGGGAUcugcuacaucgccUGGCAgauacacucgaaUCugCGCAgCCCCUGCGa -3' miRNA: 3'- -CUCUA-------------ACCGU------------AGugGCGU-GGGGACGU- -5' |
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| 21851 | 3' | -56.5 | NC_004902.1 | + | 7008 | 1.08 | 0.000526 |
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Target: 5'- uGAGAUUGGCAUCACCGCACCCCUGCAa -3' miRNA: 3'- -CUCUAACCGUAGUGGCGUGGGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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