Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21853 | 5' | -48.7 | NC_004902.1 | + | 17191 | 0.66 | 0.931116 |
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Target: 5'- uUGAcUGGCGgg-GCAUCUgcacccuuAGUGGCa -3' miRNA: 3'- cAUUuACCGUauaCGUAGG--------UCACCGc -5' |
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| 21853 | 5' | -48.7 | NC_004902.1 | + | 32704 | 0.66 | 0.918547 |
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Target: 5'- cUGAGUGGCAgg-GCucugugCCAG-GGCa -3' miRNA: 3'- cAUUUACCGUauaCGua----GGUCaCCGc -5' |
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| 21853 | 5' | -48.7 | NC_004902.1 | + | 31760 | 0.67 | 0.889569 |
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Target: 5'- --cAAUGGCAcuuauUGUGCAg-CAGUGGUa -3' miRNA: 3'- cauUUACCGU-----AUACGUagGUCACCGc -5' |
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| 21853 | 5' | -48.7 | NC_004902.1 | + | 7658 | 0.68 | 0.855712 |
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Target: 5'- -----aGGCAgcAUGCGUuugCCAGUGGCc -3' miRNA: 3'- cauuuaCCGUa-UACGUA---GGUCACCGc -5' |
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| 21853 | 5' | -48.7 | NC_004902.1 | + | 1103 | 0.68 | 0.837107 |
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Target: 5'- aUGGAUGGCAc--GUAUCCGgGUGGgGg -3' miRNA: 3'- cAUUUACCGUauaCGUAGGU-CACCgC- -5' |
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| 21853 | 5' | -48.7 | NC_004902.1 | + | 9053 | 0.69 | 0.827421 |
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Target: 5'- ---cAUGGCAUgAUGCucggCguGUGGCGa -3' miRNA: 3'- cauuUACCGUA-UACGua--GguCACCGC- -5' |
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| 21853 | 5' | -48.7 | NC_004902.1 | + | 29517 | 0.69 | 0.817495 |
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Target: 5'- -----aGGCAc--GCAUCUAGUGGUGc -3' miRNA: 3'- cauuuaCCGUauaCGUAGGUCACCGC- -5' |
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| 21853 | 5' | -48.7 | NC_004902.1 | + | 7939 | 1.11 | 0.00239 |
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Target: 5'- gGUAAAUGGCAUAUGCAUCCAGUGGCGg -3' miRNA: 3'- -CAUUUACCGUAUACGUAGGUCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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