Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21855 | 3' | -56.2 | NC_004902.1 | + | 231 | 0.66 | 0.62054 |
Target: 5'- -gGCGCU-ACAUCGGUa--GCGGGCGc -3' miRNA: 3'- ugCGUGGuUGUAGCCAgucCGCCUGU- -5' |
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21855 | 3' | -56.2 | NC_004902.1 | + | 2667 | 0.66 | 0.576185 |
Target: 5'- cCGCGCCuGCGaaUCGGUCGuuGCGGAg- -3' miRNA: 3'- uGCGUGGuUGU--AGCCAGUc-CGCCUgu -5' |
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21855 | 3' | -56.2 | NC_004902.1 | + | 14563 | 0.67 | 0.500663 |
Target: 5'- cCGCAuCCAcuaACAUUGGaCGGGCaGACAc -3' miRNA: 3'- uGCGU-GGU---UGUAGCCaGUCCGcCUGU- -5' |
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21855 | 3' | -56.2 | NC_004902.1 | + | 2520 | 0.68 | 0.479862 |
Target: 5'- aGCGaGCCAaaaACAUCGGU--GGCGGugAg -3' miRNA: 3'- -UGCgUGGU---UGUAGCCAguCCGCCugU- -5' |
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21855 | 3' | -56.2 | NC_004902.1 | + | 10854 | 0.68 | 0.473704 |
Target: 5'- aGCaCACCGGCAcagauacaggacuuuUUGGUCcagguccaAGGCGGGCAa -3' miRNA: 3'- -UGcGUGGUUGU---------------AGCCAG--------UCCGCCUGU- -5' |
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21855 | 3' | -56.2 | NC_004902.1 | + | 3454 | 0.7 | 0.374041 |
Target: 5'- cCGcCGCCGAUAUCaacgGGaUCAGGCGGAaCAa -3' miRNA: 3'- uGC-GUGGUUGUAG----CC-AGUCCGCCU-GU- -5' |
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21855 | 3' | -56.2 | NC_004902.1 | + | 38255 | 0.71 | 0.331451 |
Target: 5'- cGCGCACCAGCAccUCGccagcGGCGGGCc -3' miRNA: 3'- -UGCGUGGUUGU--AGCcagu-CCGCCUGu -5' |
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21855 | 3' | -56.2 | NC_004902.1 | + | 8473 | 1.08 | 0.000676 |
Target: 5'- aACGCACCAACAUCGGUCAGGCGGACAa -3' miRNA: 3'- -UGCGUGGUUGUAGCCAGUCCGCCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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