Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21856 | 5' | -52.3 | NC_004902.1 | + | 36680 | 0.66 | 0.812914 |
Target: 5'- cGAagGCCUacaaacaaGGCGGCGUggcagUAGCUACUg -3' miRNA: 3'- aCUa-UGGG--------UCGCCGCAa----GUUGAUGGu -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 43840 | 0.66 | 0.792926 |
Target: 5'- -cGUACgCAGCGGUGcgCG-CUGCCu -3' miRNA: 3'- acUAUGgGUCGCCGCaaGUuGAUGGu -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 12993 | 0.66 | 0.782651 |
Target: 5'- ---gACCCAGCaGGUGUUguGC-ACCGu -3' miRNA: 3'- acuaUGGGUCG-CCGCAAguUGaUGGU- -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 7060 | 0.66 | 0.782651 |
Target: 5'- cGGUGCCauccuGGCGGCGUUgCAAUacaGCCc -3' miRNA: 3'- aCUAUGGg----UCGCCGCAA-GUUGa--UGGu -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 14056 | 0.67 | 0.750856 |
Target: 5'- uUGAUGCUCAGaCaGUGacaUUAACUGCCAg -3' miRNA: 3'- -ACUAUGGGUC-GcCGCa--AGUUGAUGGU- -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 6250 | 0.67 | 0.750856 |
Target: 5'- cGAUugACUgGGCGGCGaUUgAGCUcGCCAa -3' miRNA: 3'- aCUA--UGGgUCGCCGC-AAgUUGA-UGGU- -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 41709 | 0.69 | 0.626791 |
Target: 5'- ---cGCUCuaAGCGGCGUUCcuaggcaucaccAGCUACCAc -3' miRNA: 3'- acuaUGGG--UCGCCGCAAG------------UUGAUGGU- -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 16530 | 0.69 | 0.615306 |
Target: 5'- gGAUACCUGGCGaGCua--AGCUACCGu -3' miRNA: 3'- aCUAUGGGUCGC-CGcaagUUGAUGGU- -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 561 | 0.71 | 0.492124 |
Target: 5'- -cAUACCCAGCGGCug-CAAUUGCg- -3' miRNA: 3'- acUAUGGGUCGCCGcaaGUUGAUGgu -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 9525 | 0.74 | 0.363269 |
Target: 5'- aGAUACCCAGCGGCuGUggGAUUcuacaggcccaACCAa -3' miRNA: 3'- aCUAUGGGUCGCCG-CAagUUGA-----------UGGU- -5' |
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21856 | 5' | -52.3 | NC_004902.1 | + | 9561 | 1.09 | 0.001352 |
Target: 5'- aUGAUACCCAGCGGCGUUCAACUACCAu -3' miRNA: 3'- -ACUAUGGGUCGCCGCAAGUUGAUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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