Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21857 | 5' | -57.3 | NC_004902.1 | + | 21485 | 0.66 | 0.408224 |
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Target: 5'- uGGcGUCAGCAugucUGGUAGCugggGGCGGAAu -3' miRNA: 3'- uUC-CGGUCGUu---ACCGUCG----UCGCCUUu -5' |
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| 21857 | 5' | -57.3 | NC_004902.1 | + | 14864 | 0.66 | 0.408224 |
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Target: 5'- cAGGCgCAuaugguGCAGUGGCGGCAacuGCGGu-- -3' miRNA: 3'- uUCCG-GU------CGUUACCGUCGU---CGCCuuu -5' |
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| 21857 | 5' | -57.3 | NC_004902.1 | + | 20292 | 0.68 | 0.34309 |
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Target: 5'- uGGGCCuauauuaAGUggUuGCGGCGGCGGAu- -3' miRNA: 3'- uUCCGG-------UCGuuAcCGUCGUCGCCUuu -5' |
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| 21857 | 5' | -57.3 | NC_004902.1 | + | 3879 | 0.68 | 0.335387 |
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Target: 5'- -uGGCCAuCAGUccGGCGGUAGCGGuAAg -3' miRNA: 3'- uuCCGGUcGUUA--CCGUCGUCGCCuUU- -5' |
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| 21857 | 5' | -57.3 | NC_004902.1 | + | 31768 | 0.68 | 0.310632 |
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Target: 5'- gAAGG-CAGCAAUGGCacuuauugugcAGCAGUGGu-- -3' miRNA: 3'- -UUCCgGUCGUUACCG-----------UCGUCGCCuuu -5' |
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| 21857 | 5' | -57.3 | NC_004902.1 | + | 12203 | 0.69 | 0.279824 |
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Target: 5'- cAGGCCGGCAua-GUAGUucuuGGCGGAAAc -3' miRNA: 3'- uUCCGGUCGUuacCGUCG----UCGCCUUU- -5' |
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| 21857 | 5' | -57.3 | NC_004902.1 | + | 38872 | 0.71 | 0.211717 |
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Target: 5'- uAAGGCCAuacuucgcaagacgGCAAagacuUGGCAGCacuacAGCGGAGAc -3' miRNA: 3'- -UUCCGGU--------------CGUU-----ACCGUCG-----UCGCCUUU- -5' |
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| 21857 | 5' | -57.3 | NC_004902.1 | + | 11092 | 1.03 | 0.000717 |
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Target: 5'- cAAGGCCAGCAAUGGCAGCAGCGGAAAc -3' miRNA: 3'- -UUCCGGUCGUUACCGUCGUCGCCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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