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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21859 | 3' | -49.1 | NC_004902.1 | + | 13340 | 0.66 | 0.93047 |
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Target: 5'- gGAUCgcaaaUGAGUGaguUACCuaGUUACUGCCa -3' miRNA: 3'- aCUAGa----GCUCAU---GUGG--UAAUGACGGg -5' |
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| 21859 | 3' | -49.1 | NC_004902.1 | + | 27802 | 0.67 | 0.917436 |
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Target: 5'- cGGUgaCGGGUgguuagaACACCAgggaUACaUGCCCg -3' miRNA: 3'- aCUAgaGCUCA-------UGUGGUa---AUG-ACGGG- -5' |
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| 21859 | 3' | -49.1 | NC_004902.1 | + | 17467 | 0.67 | 0.917436 |
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Target: 5'- uUGAUC-CGAuagACgGCCAgauguauUUACUGCCCu -3' miRNA: 3'- -ACUAGaGCUca-UG-UGGU-------AAUGACGGG- -5' |
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| 21859 | 3' | -49.1 | NC_004902.1 | + | 24183 | 0.67 | 0.904468 |
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Target: 5'- uUGAUCUuuaUGAucuCGCCGUUugUGCCa -3' miRNA: 3'- -ACUAGA---GCUcauGUGGUAAugACGGg -5' |
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| 21859 | 3' | -49.1 | NC_004902.1 | + | 3443 | 0.68 | 0.873617 |
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Target: 5'- gUGAUCUUG-GUAuCAUCAUcagcgacacaggUGCUGCCg -3' miRNA: 3'- -ACUAGAGCuCAU-GUGGUA------------AUGACGGg -5' |
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| 21859 | 3' | -49.1 | NC_004902.1 | + | 39720 | 0.68 | 0.873617 |
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Target: 5'- aGAUCcaaaUCGcAGgcUACCAUaACUGCCUu -3' miRNA: 3'- aCUAG----AGC-UCauGUGGUAaUGACGGG- -5' |
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| 21859 | 3' | -49.1 | NC_004902.1 | + | 37136 | 0.68 | 0.85648 |
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Target: 5'- gUGGUCUaCGAcaaauGUGCAuCCGUacccgUACUGCUCa -3' miRNA: 3'- -ACUAGA-GCU-----CAUGU-GGUA-----AUGACGGG- -5' |
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| 21859 | 3' | -49.1 | NC_004902.1 | + | 13953 | 1.14 | 0.001493 |
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Target: 5'- uUGAUCUCGAGUACACCAUUACUGCCCg -3' miRNA: 3'- -ACUAGAGCUCAUGUGGUAAUGACGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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