miRNA: 3'- agUCCAUUACa-UUACCauuuGAGAUC- -5'
miRNA: 3'- aGUCCAuua---CAUUACCAUuUGAGaUC- -5'
miRNA: 3'- aGUCCA---UUACAuUACCAU---UUGAGAUC- -5'
miRNA: 3'- aGUCCAUUAC-AU-UACCAUUUGAGAUc -5'
miRNA: 3'- -AGUCCAUUACAUUACCAUUUGAGAUC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21859 | 5' | -41.6 | NC_004902.1 | + | 35054 | 0.67 | 0.999901 |
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Target: 5'- -gAGGUAGUGccGGUGGagu-CUCUGGa -3' miRNA: 3'- agUCCAUUACa-UUACCauuuGAGAUC- -5' |
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| 21859 | 5' | -41.6 | NC_004902.1 | + | 13877 | 0.68 | 0.999462 |
|
Target: 5'- aCAGGUcgggcaGUAAUGGUGuACUCgAGa -3' miRNA: 3'- aGUCCAuua---CAUUACCAUuUGAGaUC- -5' |
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| 21859 | 5' | -41.6 | NC_004902.1 | + | 1268 | 0.68 | 0.999462 |
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Target: 5'- aCGGGUuggAAUGU-GUGGUAucuGGCUUUGGc -3' miRNA: 3'- aGUCCA---UUACAuUACCAU---UUGAGAUC- -5' |
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| 21859 | 5' | -41.6 | NC_004902.1 | + | 9950 | 0.84 | 0.530855 |
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Target: 5'- cCGGGUGAUGcUGcAUGGUAAGCUCUAc -3' miRNA: 3'- aGUCCAUUAC-AU-UACCAUUUGAGAUc -5' |
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| 21859 | 5' | -41.6 | NC_004902.1 | + | 13918 | 1.13 | 0.013956 |
|
Target: 5'- cUCAGGUAAUGUAAUGGUAAACUCUAGa -3' miRNA: 3'- -AGUCCAUUACAUUACCAUUUGAGAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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