Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2186 | 5' | -56.2 | NC_001396.1 | + | 3478 | 0.66 | 0.166368 |
Target: 5'- --aGGAuucccCGGUUGCagaaGCCAcGCAGCCUa -3' miRNA: 3'- gcaCCUu----GCCAACG----CGGU-UGUCGGGg -5' |
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2186 | 5' | -56.2 | NC_001396.1 | + | 1951 | 0.66 | 0.156184 |
Target: 5'- -cUGGGGCGGUgaaucugccgGCGCCGGCcuuaaguuGCgCCa -3' miRNA: 3'- gcACCUUGCCAa---------CGCGGUUGu-------CGgGG- -5' |
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2186 | 5' | -56.2 | NC_001396.1 | + | 4004 | 0.67 | 0.128937 |
Target: 5'- -cUGGAGCGGcacagaGCaUCAGCAGCaCCCa -3' miRNA: 3'- gcACCUUGCCaa----CGcGGUUGUCG-GGG- -5' |
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2186 | 5' | -56.2 | NC_001396.1 | + | 5906 | 0.69 | 0.095901 |
Target: 5'- -aUGG-AUGaUUGCGCCAgcucgagugcaggGCGGCCCCc -3' miRNA: 3'- gcACCuUGCcAACGCGGU-------------UGUCGGGG- -5' |
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2186 | 5' | -56.2 | NC_001396.1 | + | 3885 | 1.11 | 2.9e-05 |
Target: 5'- gCGUGGAACGGUUGCGCCAACAGCCCCc -3' miRNA: 3'- -GCACCUUGCCAACGCGGUUGUCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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