Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21861 | 3' | -54.4 | NC_004902.1 | + | 18299 | 0.66 | 0.655657 |
Target: 5'- -uGCGC-UGCUG-AGCCUC-UgCUGCc -3' miRNA: 3'- auCGUGcACGACaUCGGAGuAgGACG- -5' |
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21861 | 3' | -54.4 | NC_004902.1 | + | 190 | 0.68 | 0.530777 |
Target: 5'- aAGCaaGCGaaucUGCUGUGGCgCU--UCCUGCg -3' miRNA: 3'- aUCG--UGC----ACGACAUCG-GAguAGGACG- -5' |
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21861 | 3' | -54.4 | NC_004902.1 | + | 37076 | 0.71 | 0.377557 |
Target: 5'- cAGCGCGUGCUGUggaucgGGCC-CAUUCg-- -3' miRNA: 3'- aUCGUGCACGACA------UCGGaGUAGGacg -5' |
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21861 | 3' | -54.4 | NC_004902.1 | + | 17649 | 1.11 | 0.000515 |
Target: 5'- gUAGCACGUGCUGUAGCCUCAUCCUGCa -3' miRNA: 3'- -AUCGUGCACGACAUCGGAGUAGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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