Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21862 | 3' | -52.3 | NC_004902.1 | + | 34291 | 0.66 | 0.753122 |
Target: 5'- uAGGGcuUUGGuGCUGuCAGUGCAucAGUa- -3' miRNA: 3'- -UUCC--AACCuCGACuGUCACGU--UCAcu -5' |
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21862 | 3' | -52.3 | NC_004902.1 | + | 831 | 0.66 | 0.742083 |
Target: 5'- cAAGGUUGGugauguAGCUGGCuGUGUGAaUGGc -3' miRNA: 3'- -UUCCAACC------UCGACUGuCACGUUcACU- -5' |
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21862 | 3' | -52.3 | NC_004902.1 | + | 32859 | 0.7 | 0.48906 |
Target: 5'- uGGGUcGGAGCuguauuaccagauUGACAGguaUGCAGGUGGc -3' miRNA: 3'- uUCCAaCCUCG-------------ACUGUC---ACGUUCACU- -5' |
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21862 | 3' | -52.3 | NC_004902.1 | + | 18076 | 1.07 | 0.00151 |
Target: 5'- aAAGGUUGGAGCUGACAGUGCAAGUGAg -3' miRNA: 3'- -UUCCAACCUCGACUGUCACGUUCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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