Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21863 | 3' | -55.1 | NC_004902.1 | + | 35632 | 0.66 | 0.666185 |
Target: 5'- uCCGUGCUGGUGCAc--UCUGGCa-- -3' miRNA: 3'- cGGCACGGUCACGUuccAGACUGagu -5' |
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21863 | 3' | -55.1 | NC_004902.1 | + | 21027 | 0.66 | 0.609865 |
Target: 5'- uGCCGUGCCGaaaucucuGcUGCAAGGUCagagGAUg-- -3' miRNA: 3'- -CGGCACGGU--------C-ACGUUCCAGa---CUGagu -5' |
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21863 | 3' | -55.1 | NC_004902.1 | + | 32690 | 0.69 | 0.469451 |
Target: 5'- uCUGUGCCAGggcaauacaagacggGCGAGGaggUGACUCAg -3' miRNA: 3'- cGGCACGGUCa--------------CGUUCCag-ACUGAGU- -5' |
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21863 | 3' | -55.1 | NC_004902.1 | + | 18668 | 1.11 | 0.0005 |
Target: 5'- cGCCGUGCCAGUGCAAGGUCUGACUCAg -3' miRNA: 3'- -CGGCACGGUCACGUUCCAGACUGAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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