Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21865 | 5' | -50.6 | NC_004902.1 | + | 5119 | 0.66 | 0.898867 |
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Target: 5'- aUGGCAGauauuUAUGUUGCCcgUACuGGGAu -3' miRNA: 3'- aGCCGUUc----AUACAAUGGa-GUGuCCCU- -5' |
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| 21865 | 5' | -50.6 | NC_004902.1 | + | 42074 | 0.66 | 0.858729 |
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Target: 5'- gUUGGUggGU--GUUGCUUCAguGGGu -3' miRNA: 3'- -AGCCGuuCAuaCAAUGGAGUguCCCu -5' |
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| 21865 | 5' | -50.6 | NC_004902.1 | + | 39625 | 0.67 | 0.84988 |
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Target: 5'- aCGGCAAGggcc-UGCCaagGCAGGGAg -3' miRNA: 3'- aGCCGUUCauacaAUGGag-UGUCCCU- -5' |
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| 21865 | 5' | -50.6 | NC_004902.1 | + | 6841 | 0.69 | 0.704612 |
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Target: 5'- gUCGGUGAGUGguaUGCCa-ACAGGGAg -3' miRNA: 3'- -AGCCGUUCAUacaAUGGagUGUCCCU- -5' |
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| 21865 | 5' | -50.6 | NC_004902.1 | + | 29620 | 0.73 | 0.521103 |
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Target: 5'- gCGGCAGGUGUGccAUCUgC-CAGGGAc -3' miRNA: 3'- aGCCGUUCAUACaaUGGA-GuGUCCCU- -5' |
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| 21865 | 5' | -50.6 | NC_004902.1 | + | 23577 | 1.1 | 0.001897 |
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Target: 5'- uUCGGCAAGUAUGUUACCUCACAGGGAc -3' miRNA: 3'- -AGCCGUUCAUACAAUGGAGUGUCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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