Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21867 | 5' | -52 | NC_004902.1 | + | 40482 | 0.66 | 0.841848 |
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Target: 5'- gCCCAGCUUgauaCGUGCACGcCA---GCGCu -3' miRNA: 3'- -GGGUUGAA----GCACGUGUcGUaccUGCG- -5' |
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| 21867 | 5' | -52 | NC_004902.1 | + | 37539 | 0.66 | 0.81376 |
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Target: 5'- uCUCGACUUUG---ACAGUAaGGACGCu -3' miRNA: 3'- -GGGUUGAAGCacgUGUCGUaCCUGCG- -5' |
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| 21867 | 5' | -52 | NC_004902.1 | + | 35268 | 0.66 | 0.810845 |
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Target: 5'- gCCCAguuccaguaguuugGCUgCGUGCcUGGCGUGGA-GCu -3' miRNA: 3'- -GGGU--------------UGAaGCACGuGUCGUACCUgCG- -5' |
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| 21867 | 5' | -52 | NC_004902.1 | + | 12994 | 0.68 | 0.708711 |
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Target: 5'- aCCCAGCaggugUUGUGCACcGUggugaaaucguGUGGuCGCa -3' miRNA: 3'- -GGGUUGa----AGCACGUGuCG-----------UACCuGCG- -5' |
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| 21867 | 5' | -52 | NC_004902.1 | + | 20525 | 0.69 | 0.663733 |
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Target: 5'- aCCucuACUUCGUGCuCAGCAguguaugccUGGGCc- -3' miRNA: 3'- gGGu--UGAAGCACGuGUCGU---------ACCUGcg -5' |
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| 21867 | 5' | -52 | NC_004902.1 | + | 25444 | 1.15 | 0.000712 |
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Target: 5'- aCCCAACUUCGUGCACAGCAUGGACGCu -3' miRNA: 3'- -GGGUUGAAGCACGUGUCGUACCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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