Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 21870 | 5' | -53.6 | NC_004902.1 | + | 9246 | 0.67 | 0.658207 |
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Target: 5'- -aGUAGCuggaGAUGCGGGCCAuCGGgUCCGa -3' miRNA: 3'- ucUAUUG----CUGUGUCCGGU-GUCgAGGC- -5' |
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| 21870 | 5' | -53.6 | NC_004902.1 | + | 12410 | 0.68 | 0.624156 |
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Target: 5'- ---aGACGACGCAGGCaaugGCGGCUg-- -3' miRNA: 3'- ucuaUUGCUGUGUCCGg---UGUCGAggc -5' |
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| 21870 | 5' | -53.6 | NC_004902.1 | + | 40740 | 0.69 | 0.533293 |
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Target: 5'- aGGAUAugGcccugcaacacugGCACAGGCUgaGGCUCUGu -3' miRNA: 3'- -UCUAUugC-------------UGUGUCCGGugUCGAGGC- -5' |
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| 21870 | 5' | -53.6 | NC_004902.1 | + | 6352 | 0.7 | 0.501818 |
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Target: 5'- uGGUuGCcACcCAGGCUAUGGCUCCGg -3' miRNA: 3'- uCUAuUGcUGuGUCCGGUGUCGAGGC- -5' |
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| 21870 | 5' | -53.6 | NC_004902.1 | + | 29559 | 0.71 | 0.449603 |
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Target: 5'- cAGAUGGC-ACACcuGCCGCccuguAGCUCCGa -3' miRNA: 3'- -UCUAUUGcUGUGucCGGUG-----UCGAGGC- -5' |
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| 21870 | 5' | -53.6 | NC_004902.1 | + | 35292 | 1.1 | 0.000941 |
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Target: 5'- uAGAUAACGACACAGGCCACAGCUCCGg -3' miRNA: 3'- -UCUAUUGCUGUGUCCGGUGUCGAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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