Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
21871 | 3' | -55.7 | NC_004902.1 | + | 27673 | 0.66 | 0.619634 |
Target: 5'- gCGCaGGCGGCcCGuugauauuauGGCCUGgAAGCAa -3' miRNA: 3'- -GCG-CCGUCGcGCuu--------CCGGAUgUUUGU- -5' |
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21871 | 3' | -55.7 | NC_004902.1 | + | 11103 | 0.68 | 0.457378 |
Target: 5'- gGCGGCGuaGCcAAGGCCaGCAAugGCAg -3' miRNA: 3'- gCGCCGUcgCGcUUCCGGaUGUU--UGU- -5' |
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21871 | 3' | -55.7 | NC_004902.1 | + | 40898 | 0.72 | 0.282432 |
Target: 5'- aGCGGCuGCGCu--GGCCgcACAAGCGa -3' miRNA: 3'- gCGCCGuCGCGcuuCCGGa-UGUUUGU- -5' |
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21871 | 3' | -55.7 | NC_004902.1 | + | 36691 | 1.09 | 0.000614 |
Target: 5'- cCGCGGCAGCGCGAAGGCCUACAAACAa -3' miRNA: 3'- -GCGCCGUCGCGCUUCCGGAUGUUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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